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Plasmid#228351PurposeConstitutive ALX-0081 expressionDepositorInsertMFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
UseAffinity Reagent/ AntibodyTagsAAA linker followed by FLAG-His6 and MF_ secretio…ExpressionYeastMutationPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1461
Plasmid#228319PurposeDoxycycline-regulatable ALX-0081 expressionDepositorInsertMFalpha(2xAdv)-ALX-0081-3A-FLAG-His
UseAffinity Reagent/ AntibodyTagsAAA linker followed by FLAG-His6 and MF_ secretio…ExpressionYeastMutationPromoterDoxycycline-regulatable synthetic promoter (KpTc7…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Pchi-Q61A
Plasmid#231520PurposeExpresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generationDepositorInsertPchi HMG Q61A flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutation at position 61 Q to A of the HMG d…PromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Pchi-Q61A/E64M
Plasmid#231521PurposeExpresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generationDepositorInsertPchi HMG Q61A/E64M flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutations at position 61 Q to A and 64 E to…PromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-MjCouRS
Plasmid#229066PurposetRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codonDepositorInsertMethanococcus jannaschii tRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-marmoset cGASdeltaN-HA
Plasmid#186863PurposeDoxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transductionDepositorInsertMarmoset cGAS truncation mutant (160-547 a.a.) with C-terminal HA tag
UseRetroviralTagsHAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-WHG cGASdeltaN-HA
Plasmid#186868PurposeDoxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transductionDepositorInsertWhite-handed gibbon cGAS truncation mutant (193-557 a.a.) with C-terminal HA tag
UseRetroviralTagsHAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-W
Plasmid#208552PurposeLentiviral vector expressing gRNA targeting human RUNX3DepositorInsertRUNX3(13) (RUNX3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RUNX3(12))-PGKpuro2ABFP-W
Plasmid#208551PurposeLentiviral vector expressing gRNA targeting human RUNX3DepositorInsertRUNX3(12) (RUNX3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-W
Plasmid#210612PurposeLentiviral vector expressing gRNA targeting a control region at the CXCR4 locusDepositorInsertControl(1)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-W
Plasmid#200491PurposeLentiviral vector expressing gRNA targeting human RIC8ADepositorInsertRIC8A(16) (RIC8A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-W
Plasmid#200490PurposeLentiviral vector expressing gRNA targeting human RIC8ADepositorInsertRIC8A(15) (RIC8A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W
Plasmid#210613PurposeLentiviral vector expressing gRNA targeting a control region at the CXCR4 locusDepositorInsertControl(2)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENP-O-GFP
Plasmid#205178PurposeExpresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorInsertCENP-O (Cenpo Mouse)
UseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationWild type mouse CENP-OPromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LYK5_At-3xHA
Plasmid#202192PurposeExpress Arabidopsis thaliana LYK5 gene under UBQ10 promoterDepositorInsertLYSM-CONTAINING RECEPTOR-LIKE KINASE 5 (LYK5 Mustard Weed)
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX FLAG-SLC38A9.1
Plasmid#199749PurposeLentiviral construct to stably express SLC38A91.1DepositorInsertSLC38A9.1
UseTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ control-FLAG
Plasmid#199678PurposeBacterial expression of Leucine Zipper (LZ) control-FlagDepositorInsertLeucine zipper control
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FusionRed
Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL6-Citrine:NOSt
Plasmid#175568PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-6DepositorInsertGOXL6
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
L2_2xNOP1gRNA-Cas9-CsA
Plasmid#136140PurposePlasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygRDepositorInsertp5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only