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pUCHR-inLuc

Plasmid

#58955

Purpose

HIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.

Depositor

David Derse, Dmitriy Mazurov

Publication

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Lentiviral

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mCherry

Plasmid

#62632

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Publication

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
Mouse Alpha2 delta-2 HA pMT2

Plasmid

#58731

Purpose

expression of HA tagged mouse Alpha2 delta-2 in mamalian cells

Depositor

Annette Dolphin

Publication

Davies et al J Neurosci. 2006 Aug 23;26(34):8748-57.

Insert

alpha2 delta-2 (Cacna2d2 Mouse)

Expression

Mammalian

Promoter

SV40

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEX-CMV-SP-STIM2(15-746)

Plasmid

#18868

Depositor

Tobias Meyer

Publication

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

STIM2 (STIM2 Human)

Expression

Mammalian

Mutation

Signal peptide(SP) 1-10 from STIM1 and residues 1…

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSM1959

Plasmid

#41837

Depositor

Susan Michaelis

Publication

Metzger et al J Biol Chem. 2008 Nov 21;283(47):32302-1

Insert

SEC63-mRFP

Tags

mRFP

Expression

Yeast

Available since

Jan. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AdML

Plasmid

#11242

Depositor

Robin Reed

Publication

Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

AdML

Expression

Bacterial

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

Plasmid

#214875

Purpose

Empty lentiviral backbone for guide array cloning

Depositor

Stanley Qi

Publication

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His-SCov2-nsp7

Plasmid

#154757

Purpose

Expression of SARS-CoV-2 nsp7

Depositor

Patrick Cramer

Publication

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDRf1-GW

Plasmid

#36026

Depositor

Wolf Frommer, Dominique Loque

Publication

Loque et al Nature. 2007 Mar 8;446(7132):195-8. Epub

Type

Empty backbone

Expression

Yeast

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSVL(D2m)

Plasmid

#29336

Depositor

John Taylor

Publication

Kuo et al J Virol. 1989 May . 63(5):1945-50.

Insert

Dimer of HDV genome with 2 nucleotide deletion in delta antigen

Expression

Mammalian

Mutation

Contains a dimer of the complete HDV genome excep…

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TOM20_Halo_T2A_EGFP

Plasmid

#135443

Purpose

Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

Depositor

Kai Johnsson

Publication

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

TOM20-HaloTag7 and EGFP

Tags

TOM20

Expression

Mammalian

Promoter

CMV/TetO

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-NPM NESM

Plasmid

#13282

Depositor

Xin Wang

Publication

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEF-Bos-FLAG MDA5 MIII

Plasmid

#52876

Purpose

Expresses MDA5 with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Publication

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

IFIH1 (IFIH1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

T488A S490A

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pM-CBE

Plasmid

#196293

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding CBE in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp7 C186A-Flag

Plasmid

#11816

Depositor

Guy Salvesen

Publication

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 7 catalytic mutant (CASP7 Human)

Tags

Flag

Expression

Mammalian

Mutation

C186A

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
SAP145

Plasmid

#11239

Depositor

Robin Reed

Publication

Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Insert

SAP145 (SF3B2 Human)

Expression

Bacterial

Mutation

First 7 amino acids are missing compared to NP_00…

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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anti-Notch2_B9-pBIOCAM5

Plasmid

#39348

Depositor

John McCafferty

Publication

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch2 (clone B9) (NOTCH2, Notch2 Mouse, Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pXP422

Plasmid

#26845

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Publication

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pRSV-p65

Plasmid

#106453

Purpose

expression of NF-kB p65

Depositor

Georg Weber

Publication

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65 (RELA Human)

Expression

Mammalian

Mutation

WT

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAM1573

Plasmid

#40239

Depositor

Susan Golden

Publication

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

Cm Resistance, neutral site II integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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