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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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UAS:Zebrabow-B

Plasmid

#118216

Purpose

For broad gal4 inducible expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFCcGi

Plasmid

#59324

Purpose

Inducible expression of GFP and constitutive expression of mCherry

Depositor

Sophie Helaine, David Holden

Article

Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

Inserts

mCherry
EGFP

Expression

Bacterial

Promoter

Pbad and rpsM

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Article

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDA056

Plasmid

#131387

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Expression

Mammalian

Promoter

TRE3G

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDA077

Plasmid

#131388

Purpose

L1RP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Klentaq

Plasmid

#157862

Purpose

Plasmid for the aTc-inducible expression of the Klentaq

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

Klentaq

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDA083

Plasmid

#131389

Purpose

eGFP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

eGFP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1-6His-DHX9

Plasmid

#211440

Purpose

generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

Depositor

Frank Grosse, Helmut Pospiech

Article

Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

Insert

DHX9 (DHX9 Human)

Tags

6xHis

Expression

Insect

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSIN4-LSM14A

Plasmid

#240022

Purpose

Expresses human LSM14A fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

LSM14A

Use

Lentiviral

Tags

3X Flag

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLPO2

Plasmid

#209976

Purpose

Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (SH71_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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UAS:Zebrabow-V

Plasmid

#118218

Purpose

For broad or variegated gal4 inducible expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLPO1

Plasmid

#209975

Purpose

Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-N1-Cdc45

Plasmid

#211426

Purpose

mammalian expression of human Cdc45 C-terminally fused to EGFP

Depositor

Helmut Pospiech

Article

Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

Insert

Cdc45 (CDC45 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDA007

Plasmid

#131380

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Tags

ORF2-3xFlag

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCI5-CasRx-NES-HA (pKW387)

Plasmid

#229048

Purpose

Mammalian expression of CasRx-NES-HA

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

CasRx (RfxCas13d)

Use

CRISPR

Tags

NES-HA

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pHBV-X/EnhI-Luc

Plasmid

#71418

Purpose

To see HBV X promoter(overlaped EnhanceI) activity for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV X promoter and enhancer I driving Luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV X

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSIN4-BCAT1

Plasmid

#240021

Purpose

Expresses human BCAT1 fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

BCAT1

Use

Lentiviral

Tags

Flag

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-Cdc45

Plasmid

#126878

Purpose

Expresses human Cdc45 in bacteria

Depositor

Luca Pellegrini

Article

Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

Insert

CDC45 (CDC45 Human)

Tags

No tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-v5.9

Plasmid

#155259

Purpose

Plasmid for the aTc-inducible expression of the v5.9

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

v5.9

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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