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Cntn3-Fc-His

Plasmid

#72067

Purpose

Expresses the extracellular region of the Contactin 3 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn3 (Cntn3 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Dscaml1-AP-His

Plasmid

#71946

Purpose

Expresses the extracellular region of the Dscaml1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscaml1 (Dscaml1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-AURKB 3'UTR

Plasmid

#29475

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

AURKB 3"UTR (AURKB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
CMV-10-3xFLAG-LGP2-K651E

Plasmid

#58685

Purpose

Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

Depositor

Curt Horvath

Article

Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

Insert

LGP2-K651E (DHX58 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

mutation to CTD, K651E

Promoter

CMV

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only
Jam2-AP-His

Plasmid

#71953

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Islr2.b-Fc-His

Plasmid

#72077

Purpose

Expresses the extracellular region of the Islr2.b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Islr2.b (Islr2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Islr2.b-AP-His

Plasmid

#71951

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Islr2.b (Islr2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Jam4.1-Fc-His

Plasmid

#72081

Purpose

Expresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam4.1 (Igsf5 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFB-GST-FBXO22

Plasmid

#228367

Purpose

express human FBXO22 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXO22 (FBXO22 Human)

Use

Tags

GST

Expression

Insect

Mutation

Promoter

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-His-SKP1

Plasmid

#228368

Purpose

express human SKP1 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

SKP1 (SKP1 Human)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pACE-MBP-FBXL17 (310–701)

Plasmid

#228369

Purpose

express human FBXL17 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXL17 (FBXL17 Human)

Use

Tags

MBP

Expression

Insect

Mutation

Promoter

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-FBXL17

Plasmid

#236188

Purpose

Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FLAG-FBXL17 (FBXL17 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-BACH1

Plasmid

#232269

Purpose

Expression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-FBXO22

Plasmid

#232271

Purpose

Expression of N-terminal tagged HA-FBXO22 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXO22 (FBXO22 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-BACH2

Plasmid

#232275

Purpose

Expression of FLAG-tagged BACH2 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH2 (BACH2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroQ-GTSE1-acGFP-S91, 262, 454, 724A

Plasmid

#224741

Purpose

Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Retroviral

Tags

acGFP

Expression

Mutation

Serine 91, 262, 454, 724 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroQ-GTSE1-acGFP-S91, 262, 454, 724D

Plasmid

#224742

Purpose

Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724D mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Retroviral

Tags

acGFP

Expression

Mutation

Serine 91, 262, 454, 724 Aspartate; Valine 53 Iso…

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc P

Plasmid

#221855

Purpose

Express human Calreticulin truncated P domain with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin (CALR Human)

Use

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Expresses P domain

Promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc N

Plasmid

#221854

Purpose

Express human Calreticulin truncated N domain with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin (CALR Human)

Use

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Expresses N domain

Promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc C

Plasmid

#221856

Purpose

Express human Calreticulin truncated C domain with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin (CALR Human)

Use

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Expresses C domain

Promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge