We narrowed to 17,375 results for: puro
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Plasmid#83679Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode2
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode3
Plasmid#83680Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode3
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode4
Plasmid#83681Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode4
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode5
Plasmid#83682Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode5
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode6
Plasmid#83683Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode6
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode8
Plasmid#83685Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode8
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode10
Plasmid#83687Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode10
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode11
Plasmid#83688Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode11
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode12
Plasmid#83689Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode12
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode13
Plasmid#83690Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode13
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode14
Plasmid#83691Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode14
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-T34A-GATA6-3XAU1 puro
Plasmid#72609PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged threonine34 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S33A-GATA6-3XAU1 puro
Plasmid#72610PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine33 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro
Plasmid#72611PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37DepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine33, threonine34 and serine37 to ala…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 S592A
Plasmid#61219PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterCMV/TOAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 / alpha2 EGFP pBabe puro
Plasmid#13606DepositorInsertintegrin alpha 9 / alpha 2 (ITGA9 Human)
UseRetroviralTagsGFPExpressionMammalianMutationIntegrin alpha 9 chimera containing the cytoplasm…PromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B
Plasmid#223712PurposeExpresses autophagy flux LC3B fluorescence reporterDepositorInsertLC3B (MAP1LC3B Human, Synthetic)
UseLentiviral and Synthetic BiologyTagsmCherry::EGFP::LC3BExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR
Plasmid#172982Purposecontrol & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusionDepositorInsertcontrol sgRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE
Plasmid#125256Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selectionDepositorInserthChR2(H134R)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1a-forwardAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro
Plasmid#211622PurposeRhno1 S141ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS141APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73040PurposeExpress Nluc and copGFP dual reporter in mammalian cellsDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterPGK (phosphoglycerate kinase 1)Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro
Plasmid#205631PurposeExpression of TnpBmax in mammalian cellsDepositorInsertISDra2-TnpB optimized for mammalian expression
UseTagsNLS-3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1
Plasmid#118593PurposeExpresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoterDepositorInsertPuro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Alpha/TRE3GAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE)
Plasmid#214876PurposeLentiviral vector encoding RfxCas13d nontargeting control guideDepositorInserthU6-crNT-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)
Plasmid#225756PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase
Plasmid#188391PurposeReporter for BCAM expressionDepositorInsertBCAM reporter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73022PurposeLuminescent and fluorescent dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR
Plasmid#207805PurposeE-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.DepositorInsertE-NoMi
UseLentiviralTags3xFlag tag, copGFP, mCherry, and nanoluciferaseExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE
Plasmid#138151PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR_BsmBI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
Plasmid#107270PurposeExpresses Cas9 upon induction with doxicycline.DepositorInsertshSpCas9
rtTA
bGH polyA-SV40polyA
UseTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCAG and TREAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV PIG (Puro IRES GFP empty vector)
Plasmid#21654PurposeRetroviral backbone for cloning and gene expression. Select with puromycin or GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73037PurposeExpress Nluc and copGFP dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A Δ820-870-pBabe-Puro
Plasmid#25949DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro
Plasmid#141385PurposeLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-E (E SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro
Plasmid#141391PurposeLentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-N (N SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
Plasmid#141386PurposeLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-M (M SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227APromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290
Plasmid#197989PurposeFor the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.DepositorInsertHSALNT0217290
UseLentiviralTagslacExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE
Plasmid#32703DepositorTypeEmpty backboneUseCre/Lox and RetroviralTags3x HA tagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro
Plasmid#120559PurposeExpresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertHRAS (HRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro
Plasmid#120563PurposeExpresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX
Plasmid#202606PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-1P-P2A-mCherry-CAAX
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP
Plasmid#233445PurposePiggyBac donor plasmid to overexpress a full length ADAR2DepositorInsertADAR2-T2A-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationWTPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro
Plasmid#83306PurposeLentivirus vector to express guideRNA and dCas9 with puro resistant geneDepositorInsertsgRNA and dCas9 from pX330
UseLentiviralTagsFLAG and PA tagsExpressionMammalianMutationPromoterU6 for sgRNA and CBh for dCas9Available sinceDec. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)
Plasmid#214881PurposeLentiviral vector encoding RfxCas13d targeting B2M guideDepositorInserthU6-crB2M-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag
Plasmid#132403Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR
Plasmid#208379PurposeEpisomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistanceDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhuman CMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro human-GFP-RLIM-IRES-mCherry
Plasmid#232243PurposeMammalian expression of RLIM-GFP-IRES-mCherryDepositorInsertRLIM-GFP-IRES-mCherry (RLIM Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro GFP-REX1[degron]-IRES-mCherry
Plasmid#232240PurposeMammalian expression of REX1[degron]-GFP-IRES-mCherryDepositorInsertREX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
UseTagsAcGFP1ExpressionMammalianMutationREX1 (aa 70-85)PromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only