We narrowed to 10,942 results for: NSI;

1. pLJM1-zeo G226A ORF29

   Plasmid

   #200024

   Purpose

   Lentiviral vector for expression of KSHV ORF29 with G226A mutation

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF29
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glycine 226 changed to alanine

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUBQ10:WNFLS2-2

   Plasmid

   #202190

   Purpose

   Express 'Washington navel' Orange FLS2-2 gene under the UBQ10 promoter

   Depositor

   Gitta Coaker

   Article

   Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

   Insert

   Flagellin sensing 2
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBQ10:WNFLS2-1

   Plasmid

   #202189

   Purpose

   Express 'Washington navel' Orange FLS2-1 gene under the UBQ10 promoter

   Depositor

   Gitta Coaker

   Article

   Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

   Insert

   Flagellin sensing 2
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUE1-TSP-ORF68 R14A/K310A

   Plasmid

   #162655

   Purpose

   Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acids R14 and K310 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1-zeo-2xStrep-ORF68 C52A

   Plasmid

   #162660

   Purpose

   Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Lentiviral

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acid C52 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

   Plasmid

   #162639

   Purpose

   Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acids K174, R179, and K182 mutated to alani…

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUE1-TSP-ORF68 K435A

   Plasmid

   #162651

   Purpose

   Expresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acid K435 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUE1-TSP-ORF68 K450A/R451A

   Plasmid

   #162653

   Purpose

   Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acids K450 and R451 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-Synapsin-mScarlet-optoJNKi-WPRE

   Plasmid

   #159968

   Purpose

   optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mScarlet-optoJNKi
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-Synapsin-mTurquoise2-optoJNKi-WPRE

    Plasmid

    #159966

    Purpose

    optogenetic JNK inhibitor with increased RET to LOV2 to increase actuation sensitivity

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mTurquoise2-optoJNKi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-Synapsin-Ypet-optoJNKi-WPRE

    Plasmid

    #159967

    Purpose

    optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    Ypet-optoJNKi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA4/TO-mu24 1-181-2xStrep

    Plasmid

    #138444

    Purpose

    Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-181

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA4/TO-mu24 1-191-2xStrep

    Plasmid

    #138445

    Purpose

    Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-191

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4/TO-mu24 1-216-2xStrep

    Plasmid

    #138446

    Purpose

    Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-216

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4/TO-mu24-2xStrep

    Plasmid

    #138435

    Purpose

    Expresses full-length mu24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

    Plasmid

    #153443

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ORF18(R158A_L159A)-3xFLAG pCDNA4

    Plasmid

    #129814

    Purpose

    Expresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Arginine 158 to Alanine and Leucine 159 t…

    Promoter

    CMV

    Available since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. K8.1Pr-pGL4.16-OriLyt

    Plasmid

    #131038

    Purpose

    Firefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replication

    Depositor

    Britt Glaunsinger

    Article

    Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

    Insert

    KSHV Lytic Origin of replication
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Thy1.1-muSOX D219A

    Plasmid

    #131704

    Purpose

    encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-muSOX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Aspartic acid 219 mutated to an alanine

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ORF18(G228A)-3xFLAG pCDNA4

    Plasmid

    #129809

    Purpose

    Expresses KSHV ORF18 (G228A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 228 to Alanine

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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