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Plasmid#27380DepositorInsertCep290 (Cep290 Mouse)
UseTagsmCherryExpressionMammalianMutationplease see depositor comments belowPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC
Plasmid#114377Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targetingDepositorInsertGtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
UseAAVTagsEYFP and Kv2.1C-linker-TlcnCExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pEBTet-SNAP-Cep63
Plasmid#136811PurposeMammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep63 (CEP63 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mCep290
Plasmid#27381DepositorInsertCep290 (Cep290 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8f
Plasmid#169249PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.DepositorInsertRiboL1-jGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-ALMS1
Plasmid#136877PurposeMammalian expression of the centrosomal protein ALMS1 N-terminally fused to CLIP-tagDepositorInsertCLIP-ALMS1 (ALMS1 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Cep63
Plasmid#136812PurposeMammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep63 (CEP63 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
PDI-QUAD-V5-pcDNA3.1
Plasmid#153550PurposePDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.DepositorInsertProtein disulphide isomerase
UseTagsV5 tagExpressionMutationC53S, C56S, C397S, C400SPromoterpCMVAvailable sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
RpH-ILV-FRB
Plasmid#225137PurposeRpH-ILV probe strongly colocalises with lysosomal markers with FRBDepositorInsertTMEM192 (TMEM192 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mCep290
Plasmid#27379DepositorInsertCep290 (Cep290 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep152
Plasmid#136874PurposeMammalian expression of the centrosomal protein Cep152 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep152 (CEP152 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-FLucStop
Plasmid#177617PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationIn-frame stop codon added between FLuc and FSEPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCXLE-hUL + mTAGBFP2
Plasmid#74944PurposeDosage control and tracing of reprogramming episomesDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep128
Plasmid#136872PurposeMammalian expression of the centrosomal protein Cep128 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep128 (CEP128 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcsII-pb4-flag
Plasmid#86768PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertproteasome beta subunit 4 (PSMB4 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 R2pH-LAMP1-3xFLAG
Plasmid#157941Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAG, mCherry, and pHluorinExpressionMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep97
Plasmid#136800PurposeMammalian expression of the centrosomal protein Cep97 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep97 (CEP97 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-ALMS1
Plasmid#136828PurposeMammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tagDepositorInsertSNAP-ALMS1 (ALMS1 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep78
Plasmid#136827PurposeMammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep78 (CEP78 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only