We narrowed to 24,475 results for: crispr

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pIVT-Cas9-DEN2

Plasmid

#238017

Purpose

for DNA-free gene knockout editing in rice and wheat or other plants

Depositor

Caixia Gao

Article

Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

Insert

SpCas9

Expression

Plant

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTarget12f1

Plasmid

#171183

Purpose

Constitutive expression of sgRNA without donor DNA

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

sgRNA scaffold in the CRISPR/Cas12f1 system

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI) promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#186276

Purpose

Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira shortened CRISPR array

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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AAV8-cPE-N

Plasmid

#183538

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

N-terminal fragment of SpCas9 nickase (H840A)

Use

AAV

Expression

Mammalian

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBA1739

Plasmid

#246064

Purpose

Bacterial expression of mCpol under control of the pTet promoter

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Promoter

pTet promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-miRFP670

Plasmid

#187223

Purpose

Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-mStrawberry

Plasmid

#187224

Purpose

Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

CRISPR

Tags

Inserted T2A-mStrawberry at 3' terminal of M…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x5

Plasmid

#58775

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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ED_p_060

Plasmid

#246070

Purpose

ECOR31 mCpol bacterial expression under T7 promoter, C-term TEV-MBP-HIS10

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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ED_p_044a

Plasmid

#246071

Purpose

ECOR31 mCpol bacterial expression under T7 promoter, N-term His10-MBP-TEV

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pUb-Cas9-EGFP_cU6:6-sgRNA

Plasmid

#190597

Purpose

The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

Depositor

Claudia Rueckert

Article

Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

Inserts

Cas9
sgRNA

Use

CRISPR

Tags

EGFP - separated by T2A

Promoter

Aedes aegypti polyubiquitin and Culex quinquefasc…

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-MP-84-spCas9-U6-gRNA-tlock

Plasmid

#206936

Purpose

Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

Depositor

Markus Grompe

Insert

spCas9

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Promoter

MP-84

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAH235

Plasmid

#121145

Purpose

Cas9 expression controlled by the nmt41 promoter

Depositor

Katsunori Tanaka

Article

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

humanized Streptococcus pyogenes Cas9

Use

CRISPR

Tags

Flag

Expression

Yeast

Promoter

nmt41

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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ED_p_044b

Plasmid

#246072

Purpose

ECOR31 mCpol D57A bacterial expression under T7 promoter, N-term His10-MBP-TEV

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Minimal CRISPR polymerase

Expression

Bacterial

Mutation

D57A

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

Plasmid

#126753

Purpose

Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

WT SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_047

Plasmid

#107145

Purpose

measures Cas9 activity

Depositor

David Root

Insert

GFP + sgRNA for GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRDB_181

Plasmid

#216102

Purpose

CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDF0039 DisCas7-11 only expression with CRISPR array

Plasmid

#172500

Purpose

Encodes the DisCas7-11 protein only for bacterial expression

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 only expression with CRISPR array

Expression

Bacterial

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

Plasmid

#69224

Purpose

Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS and Zif268

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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