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AiP12626 - pAAV-AiE0782m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2626)

Plasmid

#191712

Purpose

AI plasmid ALIAS: AiP12626. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12025 - pAAV-3xSP10ins-AiE0367h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2025)

Plasmid

#191716

Purpose

AI plasmid ALIAS: AiP12025. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12514 - pAAV-3xSP10ins-AiE0367h_C2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2514)

Plasmid

#191717

Purpose

AI plasmid ALIAS: AiP12514. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSL4624 (pEffector_TnpB1,ωRNA)

Plasmid

#210870

Purpose

Expresses GstTnpB1 and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstTnpB1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT26i4Gli8G4E3(#1254)

Plasmid

#184074

Purpose

cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Insert

Gal4bdFF-ERT2

Use

Zebrafish transgenesis (blue heart)

Tags

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

8x3'Gli-BS

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pexK4_4hb_straight

Plasmid

#89678

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the straight four-helix-bundle shown in figure 4A of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

4hb_straight

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pexK4_4hb_bent

Plasmid

#89679

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent four-helix-bundle shown in figure 4E of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

4hb_bent

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pexK4_bow_360

Plasmid

#89673

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent two-helix-bundle shown in figure 3B of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

bow_360

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pexA2_bow_180

Plasmid

#89671

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent two-helix-bundle shown in figure 3A of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

bow_180

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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p52sheLL

Plasmid

#46950

Purpose

HPV52 L1 and L2 Bicistronic, too large to self-package

Depositor

John Schiller

Article

Kondo et al J Med Virol. 2008 May;80(5):841-6. doi:

Insert

HPV52L1+L2 (L1, L2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pHCMV-AmphoEnv

Plasmid

#15799

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

4070A Amphotropic Murine leukemia virus envelope gene

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pLARRY-EGFP

Plasmid

#140025

Purpose

Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

Depositor

Fernando Camargo

Article

Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-KDEL

Plasmid

#226568

Purpose

Encodes mNeonGreen with KDEL ER lumenal target sequence

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

KDEL (ER lumenal target sequence)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TadCBE-Cas9NG

Plasmid

#225093

Purpose

Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al bioRxiv 2024.09.09.611392

Insert

TadCBE-Cas9NG

Use

CRISPR

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-UL32

Plasmid

#162664

Purpose

Lentiviral vector for stable expression of UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMTB

Plasmid

#112225

Purpose

Expression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.

Depositor

Sean Megason

Article

Wagner et al Science. 2018 Apr 26. pii: science.aar43

Insert

Super-folder GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

beta actin 2 (zebrafish)

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237)

Plasmid

#191707

Purpose

AI plasmid ALIAS: AiP12237. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)

Plasmid

#199777

Purpose

enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Has Service

AAV PHP.eB

Insert

iCre(R297T)

Use

AAV

Tags

Expression

Mutation

R297T for reduced recombinase efficiency.

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15578 - pAAV-AiE0780m-minBG-iCre(R297T)-BGHpA (Alias: CN5578)

Plasmid

#224200

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

iCre(R297T)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14872: pAAV-AiE0452h_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN4872)

Plasmid

#214866

Purpose

Expression of RiboL1-jGCaMP8s (soma targeted) in striatal indirect pathway medium spiny neurons (D2 MSNs)

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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