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pLV hU6-gRNA hUbC-dSpCas9-2xVP64-2A-Thy1.1

Plasmid

#253230

Purpose

Expresses gRNA and VP64-dSpCas9-VP64-2A-Thy1.1 from lentiviral vector

Depositor

Charles Gersbach

Article

Reisman et al bioRxiv 2025.10.11.681828

Insert

Humanized VP64-dSpCas9-VP64-2A-Thy1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_aCatTS_indicator

Plasmid

#252386

Purpose

Non-FRET aCatenin tension indicator. cpEGFP with elastic linker is inserted at the hinge with C-term mCherry.

Depositor

Maretoshi Hirai

Article

Fujiwara et al Commun Biol. 2026 Feb 19. doi: 10.1038/s

Insert

aCatenin tension indicator

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_aActTS_indicator

Plasmid

#252385

Purpose

Non-FRET aActinin tension indicator. cpEGFP with elastic linker is inserted at the hinge with C-term mCherry.

Depositor

Maretoshi Hirai

Article

Fujiwara et al Commun Biol. 2026 Feb 19. doi: 10.1038/s

Insert

aActinin tension indicator

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_J23102_R1_LuxR

Plasmid

#250887

Purpose

LuxR regulator protein expressed under BBa_J23102 promoter; part of BNeu AS4, AS8, AS9 and AS14; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

LuxR

Use

Synthetic Biology

Promoter

BBa_J23102

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pVG298

Plasmid

#227762

Purpose

Expresses a version of light-inducible transcription factor EL222 (Thr83Ala) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Thr83Ala mutant

Promoter

PGK1pr

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLux_R1_CI*

Plasmid

#250883

Purpose

Expresses frame-shifted cI under the control of PLux promoter; part of BNeuAS2 and BNeuAS3; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

Mutated cI

Use

Synthetic Biology

Mutation

cI* = Frame-shifted cI protein

Promoter

Plux

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1467_pTet-ECOR31Ham_hamB_walkerB_Mut

Plasmid

#223367

Purpose

Expression of ECOR31 Hachiman mutant (HamB D431A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamB D431A)

Expression

Bacterial

Mutation

HamB D431A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1469_pTet-ECOR31Ham_hamA_E138A_K140A_Mut

Plasmid

#223368

Purpose

Expression of ECOR31 Hachiman mutant (HamA E138A, K140A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamA E138A, K140A)

Expression

Bacterial

Mutation

HamA E138A, K140A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4-MS2-CP-GFP-SBP

Plasmid

#249048

Purpose

Expresses MS2 cap binding protein (CP) fused to GFP and streptavidin binding peptide (SBP)

Depositor

Jeffrey Gerst

Article

Slobodin et al RNA. 2010 Nov;16(11):2277-90. doi: 10.12

Insert

MS2 binding coat protein

Tags

GFP and streptavidin binding peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pVG286

Plasmid

#227756

Purpose

Expresses a version of light-inducible transcription factor EL222 (Gly80Glu) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Gly80Glu mutant

Promoter

PGK1pr

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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