We narrowed to 159,258 results for: addgene

1. TAL2065

   Plasmid

   #39435

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-39-Right
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

2. TAL2064

   Plasmid

   #39434

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-39-Left
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

3. p-dCas9-HDAC1-Hygro

   Plasmid

   #104409

   Purpose

   transient expression of dCas9-HDAC1 fusion protein

   Depositor

   Stephan Beck

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-PERK(LD)-EGFP

   Plasmid

   #225680

   Purpose

   Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

   Depositor

   Likun Wang

   Article

   Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

   Plasmid

   #159908

   Purpose

   Mutagenesis of Htr2a

   Depositor

   Larry Zweifel

   Insert

   Htr2a (Htr2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. DCAF1:PBC020-D05:C246337

   Plasmid

   #183974

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   DCAF1:PBC020-D05:C246337 (DCAF1 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV and MHHHHHHSSGRENLYFQG

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pTec_mCherry

   Plasmid

   #229145

   Purpose

   Promoter strength evaluation of Ptec

   Depositor

   Shrikrishnan Sankaran

   Article

   Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Ptec

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-Guide-Zeo

   Plasmid

   #127173

   Purpose

   Entry plasmid for guide cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti SpBsmBI sgRNA Hygro

   Plasmid

   #62205

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. HECTD1

    Plasmid

    #32860

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HECTD1 (HECTD1 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 1879-1966 only

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuide-mCherry

    Plasmid

    #217005

    Purpose

    lentiviral plasmid for gRNA cloning with selectable mCherry expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HO-hisG-URA3-hisG-poly-HO

    Plasmid

    #51663

    Purpose

    yeast plasmid for integration of target sequence at HO locus, URA3 selection

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAPK1

    Plasmid

    #73248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pN3-Sp3FL

    Plasmid

    #24541

    Depositor

    Guntram Suske

    Article

    Sapetschnig et al J Biol Chem. 2004 Oct 1. 279(40):42095-1

    Insert

    transcription factor Sp3 (SP3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pX458-HypaCas9

    Plasmid

    #190604

    Purpose

    PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSADdeltaG-GFP-F2

    Plasmid

    #32635

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    GFP
    

    Use

    Rabies virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNM3755

    Plasmid

    #154116

    Purpose

    tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆pes-10::GFP-C1::act-4 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. LRG2.1-CyOFP1

    Plasmid

    #124771

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6CC8 (MBD3)

    Plasmid

    #119959

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD3 (MBD3 Human)
    

    Use

    Tags

    GSTtag, 6xHis and Thrombin

    Expression

    Bacterial

    Mutation

    MBD domain (contains residues 1-71)

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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