We narrowed to 8,293 results for: construct

1. pGBW-m4133301

   Plasmid

   #152078

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   May 19, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4133294

   Plasmid

   #152067

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 endoRNAse

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;3xFlag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   May 19, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGBW-m4046635

   Plasmid

   #149149

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp9

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pYZ160

   Plasmid

   #98416

   Purpose

   Pombe Expression Vector - nmt81 promoter with Sp.leu1 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK652

   Plasmid

   #72470

   Purpose

   Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)

   Depositor

   T. J. Kappock

   Insert

   acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutated histidine-233 to glycine

   Promoter

   T7

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGEV_-1_XcmI-LacZ

   Plasmid

   #49296

   Purpose

   Golden Gate entry vector at position 1, no 5' ATG, LacZ

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGGW_SM_BglII

   Plasmid

   #49314

   Purpose

   Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3E_GGWDest+

   Plasmid

   #49317

   Purpose

   Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pME_GGWDest-

   Plasmid

   #49320

   Purpose

   Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGGEV_-1_Linker

    Plasmid

    #49281

    Purpose

    Golden Gate entry vector at position 1, small linker insert, no 5' ATG

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICH84011

    Plasmid

    #48035

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pICH83988

    Plasmid

    #48032

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pICH83999

    Plasmid

    #48033

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pICH83955

    Plasmid

    #48036

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pTKEI-Mal-B2

    Plasmid

    #79756

    Purpose

    Single fluorescent protein biosensor for maltose

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Mal-B2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTKEI-Tre-C04

    Plasmid

    #79754

    Purpose

    Single fluorescent protein biosensor for trehalose

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Tre-C04
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-LaminA-C-18

    Plasmid

    #55068

    Purpose

    Localization: Nuclear Envelope, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. spNW30

    Plasmid

    #173485

    Purpose

    Expresses spNW30 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW30
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. spNW15

    Plasmid

    #173483

    Purpose

    Expresses spNW15 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW15
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. spNW25

    Plasmid

    #173484

    Purpose

    Expresses spNW25 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW25
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge