Addgene

Showing: 1341 - 1360 of 1942 results

Results per page:

Sort results by:

pcDNA3-ApoE3

Plasmid

#197147

Purpose

Expresses ApoE3 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE3 (APOE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ApoE2

Plasmid

#197146

Purpose

Expresses ApoE2 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE2 (APOE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX313-Firefly luciferase

Plasmid

#118017

Purpose

Constitutive expression of Firefly luciferase

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ApoE4

Plasmid

#197148

Purpose

Expresses ApoE4 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE4 (APOE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_FOXA1_#1

Plasmid

#70095

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO_FOXA1_#2

Plasmid

#70096

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

u6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-PRMT6(V86K/D88A)

Plasmid

#164698

Purpose

Expression of PRMT6(V86K/D88A) with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

Insert

PRMT6 (PRMT6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

V86K, D88A

Promoter

CMV

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX313-Renilla luciferase

Plasmid

#118016

Purpose

Constitutive expression of Renilla luciferase

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Renilla luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pg-ODD-EGFP

Plasmid

#87211

Purpose

HIF-1a's ODD domain fused to EGFP

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-1a ODD domain (HIF1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

ODD domain (AA 530-652)

Promoter

CMV

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS0045

Plasmid

#131124

Purpose

Mammalian Target-ACE expression vector

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI

Use

CRISPR

Tags

PmCDA1, TadA, and UGI

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

Promoter

CMV promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-LRP6-3XFlag

Plasmid

#180144

Purpose

flag tagged LRP6

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pg-HIF-1alpha-EGFP

Plasmid

#87204

Purpose

C-terminally EGFP-tagged human HIF-1a

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-1a (HIF1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L40F, M88V, Q814R

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX NPC1-FLAG-TiD

Plasmid

#199748

Purpose

Lentiviral construct to stably express NPC1 TurboID

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

NPC1 (NPC1 Human)

Use

Tags

Flag and TurboID

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3/hArf6(WT)-HA

Plasmid

#79424

Purpose

Expresses C-terminally HA-tagged Arf6(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX311-Cas9

Plasmid

#118018

Purpose

Constitutive expression of Cas9

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Cas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-All-in-one-hCascade

Plasmid

#134919

Purpose

Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3/hArf5(WT)-mCherry

Plasmid

#79421

Purpose

Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6/V5-DEST-RINT1

Plasmid

#40124

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

RINT1 (RINT1 Human)

Use

Lentiviral

Tags

V5 epitope

Expression

Mammalian

Mutation

L270M relative to NM_021930

Promoter

CMV

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB CAG SMARCA4-Venus

Plasmid

#153950

Purpose

piggyBac transposon vector with CAG promoter expressing SMARCA4-Venus fusion protein

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 LRP6-eGFP

Plasmid

#180143

Purpose

to make movies of LRP6 vesicles

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge