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pET-28b(+)/ADK C77S

Plasmid

#201409

Purpose

Overexpresses Escherichia coli adenylate kinase C77S

Depositor

Gary Pielak

Article

Brom et al Biomol NMR Assign. 2023 Aug 26. doi: 10.

Insert

Escherichia coli adenylate kinase C77S (adk Escherichia coli)

Expression

Bacterial

Mutation

changed cysteine 77 to serine

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt8

Plasmid

#184509

Purpose

Mammalian expression of Synaptotagmin 8 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 8 (Syt8 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG1

Plasmid

#188967

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtggaaaacaatgcgaccgactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG2

Plasmid

#188968

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGP8GF-ZmAFB2/3 b2

Plasmid

#188425

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 b2

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGP8GF-ZmAFB2/3 a

Plasmid

#188423

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 A

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 a

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGP4G-REL2N91-ccdB

Plasmid

#188444

Purpose

Zea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDEST

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

REL2N91/ccdB

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-ZmIAA8ΔPB1

Plasmid

#188432

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA8

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA8deltaPB1

Use

Cloning

Mutation

deleted PB1 domain

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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GP826

Plasmid

#164551

Purpose

Expresses Smo-FLAG K444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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