Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
Type
Blog Post
...frequency of recombinants is 1 positive clone out of 104 to 105 colonies.
Pro Tip: Use a high-fidelity Taq...Insertion or deletion of >20 nucleotides
1 out of 104 to 105
Use a high fidelity Taq polymerase to generate...