We narrowed to 1,094 results for: gibson assembly
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Plasmid#239829PurposeWT OrufIscB omegaRNA mammalian expressionDepositorInsertOrufIscB omegaRNA scaffold
ExpressionMammalianPromoterhuman U6Available SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
Tags3xMS2ExpressionMammalianPromoterSV40Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJB65
Plasmid#226319PurposeE. coli protein expression for protein purificationDepositorInsert6xHis- (VTG to AAA MR1-6)-strep
Tags6xHis and StrepExpressionBacterialMutationN-terminal domain deleted, C-terminal domain deleā¦PromoterT7Available SinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-Fld
Plasmid#202181PurposeLevel 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertFld terminator
UseSynthetic BiologyMutationNoneAvailable SinceFeb. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-P49
Plasmid#202176PurposeLevel 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertP49 terminator
UseSynthetic BiologyMutationNoneAvailable SinceOct. 19, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_DE-Per_sig_pep
Plasmid#202152PurposeLevel 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.DepositorInsertPeroxisome signal peptide
UseSynthetic BiologyMutationNoneAvailable SinceOct. 19, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSC-cUnaG
Plasmid#207631PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.DepositorInsertphotocaged SpyCatcher-cUnaG
Tags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-act1_full
Plasmid#202172PurposeLevel 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertact1 full terminator
UseSynthetic BiologyMutationNoneAvailable SinceSept. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-glna
Plasmid#202183PurposeLevel 0 plasmid containing a terminator sequence from gene 22357 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertglna terminator
UseSynthetic BiologyMutationNoneAvailable SinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-act1_tr
Plasmid#202171PurposeLevel 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertact1 truncated terminator
UseSynthetic BiologyMutationNoneAvailable SinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits