Addgene

We narrowed to 12,544 results for: LIC

Showing: 3401 - 3450 of 12544 results

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6972 pPROEX-HTb-SSA1

Plasmid

#1231

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Ssa1 (SSA1 Budding Yeast)

Use

Tags

his tag (tev cleavable)

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
ancBE4-SpRY

Plasmid

#226855

Purpose

Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

ancBE4max-SpRY

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FG121VDHFR_R166QTS

Plasmid

#191298

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F/G121V DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPING

Plasmid

#26046

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

SS peptide-LacZ-KHHHHHH

Use

Tags

KHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETG

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-SRm160

Plasmid

#11305

Depositor

Phil Sharp

Article

Cheng et al Mol Cell Biol. 2006 Jan . 26(1):362-70.

Insert

SRm160 (SRRM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pKJ0377

Plasmid

#228712

Purpose

Mammalian expression of human codon-optimized enhanced Bxb1 (epBxb1 mutant T166R)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Bxb1_T166R

Use

Tags

Expression

Mammalian

Mutation

T166R

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKJ0463

Plasmid

#228715

Purpose

Bacterial expression of epT7 RNAP; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Sumo_epT7

Use

Tags

Expression

Bacterial

Mutation

T3M/G47A/E643G

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDiGi

Plasmid

#59323

Purpose

Inducible expression of dsRed and GFP in bacteria

Depositor

David Holden, Sophie Helaine

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pbad and Plac

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW357d5

Plasmid

#227931

Purpose

High-stringency accessory plasmid for selection

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Use

Tags

Expression

Bacterial

Mutation

R632S, Q649S

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINE

Plasmid

#26043

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His-lacZ-KHHHHHH

Use

Tags

KHHHHHH and MAHHHHHHSSG

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mCitrine-C1

Plasmid

#54587

Purpose

Localization: C1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Roger Tsien, Robert Campbell, Oliver Griesbeck, Michael Davidson

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSAM

Plasmid

#45174

Depositor

Jumi Shin

Article

Sathiamoorthy et al PLoS One. 2012;7(10):e47259. doi: 10.137

Type

Empty backbone

Use

Tags

6xHis and 6xHis/Thrombin/T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
pCCL-GKL1ShortMutPRC-Ug1Lg2starTGA

Plasmid

#52347

Purpose

Integration cassette to create p1-Short-UL*(TGA)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

leu*

Use

P1 integration vector

Tags

Expression

Bacterial

Mutation

changed S2F, A3V, Q180*

Promoter

UCS from pGKL2 ORF5

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Citrine-C1

Plasmid

#54715

Purpose

Localization: C1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Roger Tsien, Robert Campbell, Oliver Griesbeck, Michael Davidson

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Use

Tags

Citrine

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLV.MRE11i

Plasmid

#15565

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2006 Mar . 80(5):2445-52.

Insert

MRE11 shRNA (MRE11 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2007

Availability

Academic Institutions and Nonprofits only
ABE8.20-SpRY

Plasmid

#226859

Purpose

Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

ABE8.20-SpRY

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-30-His6-SUMO-AEY-CfaN

Plasmid

#122949

Purpose

Bacterial expression of N-intein

Depositor

Thomas Muir

Article

Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

Insert

CfaN

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUK

Plasmid

#64185

Purpose

harbours marker genes URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLK

Plasmid

#64186

Purpose

harbours marker genes LEU2 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Peredox-mCherry-NLS

Plasmid

#32385

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Use

Retroviral

Tags

Nuclear Localization Seq

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-GW FLII12Pglu-700μδ6

Plasmid

#28002

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLII12Pglu-700μδ6

Use

Tags

eCFP and eYFP

Expression

Yeast

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pSUPER.MRE11i

Plasmid

#15566

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2006 Mar . 80(5):2445-52.

Insert

MRE11 shRNA (MRE11 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2007

Availability

Academic Institutions and Nonprofits only
pPTK021-7-PARS

Plasmid

#84990

Purpose

Type 7, Pichia autonomously replicating sequence

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Pichia autonomously replicating sequence

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GW1-Peredox-mCherry-NLS

Plasmid

#32381

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Use

Tags

Nuclear Localization Seq

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
p2lox-cAMPr R739A

Plasmid

#102618

Purpose

cAMPr with a point mutation R739A which theoretically increases its affinity for cAMP

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

R739A

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-GW AT1.03YEMK

Plasmid

#28004

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03YEMK

Use

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

M60Y, G131E, K132M, 133K

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL0_62 [sfYFP]

Plasmid

#198974

Purpose

Level 0 part

Depositor

Nili Ostrov, Cultivarium Tools, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

sfYFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKFSS1_2

Plasmid

#118227

Purpose

Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR3.1-OPNa

Plasmid

#107980

Purpose

expression of full-length OPN

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

full-length osteopontin (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
RNT1-GFP

Plasmid

#17708

Depositor

Hal Dietz

Article

Mendell et al Science. 2002 Oct 11. 298(5592):419-22.

Insert

rent1 (UPF1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
RTB300

Plasmid

#80410

Purpose

human cTNT minigene with genomic segment containing alternative exon 5

Depositor

Thomas Cooper

Article

Philips et al Science. 1998 May 1;280(5364):737-41.

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

RSV

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS3805

Plasmid

#185746

Purpose

Sub-array spacer 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 1/4

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS3806

Plasmid

#185747

Purpose

Sub-array spacer 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 2/4

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEERM4 Cm

Plasmid

#64026

Purpose

Plasmid for integration into neutral site 2 of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PnrsB

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY071f

Plasmid

#227942

Purpose

SICLOPPS precursor for cyclo-CRCVFSG

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CRCVFSG

Use

Tags

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_2

Plasmid

#118225

Purpose

Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLDV_41 [11o1G]

Plasmid

#199070

Purpose

Odd assembly vector with sfGFP dropout for Odd level assemblies

Depositor

Nili Ostrov, Cultivarium Tools, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-BALF5

Plasmid

#72630

Purpose

Mammalian expression vector for Epstein-Barr virus DNA polymerase

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BALF5

Use

Tags

Expression

Mammalian

Mutation

G249S

Promoter

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
Ftz

Plasmid

#11243

Depositor

Robin Reed

Article

Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

Ftz (ftz Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pEERM3 Km

Plasmid

#64025

Purpose

Plasmid for integration into neutral site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PnrsB

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEERM1 Km

Plasmid

#64024

Purpose

Plasmid for integration into psbA2 site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PpsbA2

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH116

Plasmid

#22651

Depositor

Lynn Hudson

Article

Hudson et al Proc Natl Acad Sci U S A. 1987 Mar . 84(

Insert

Proteolipid Protein (Plp1 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
6975 pPROEX-HTb-SIS1

Plasmid

#1233

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

sis1 (SIS1 Budding Yeast)

Use

Tags

his tag (tev cleavable)

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pMOD3-proD/Lux-nat

Plasmid

#120324

Purpose

Bacterial luminescence

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL05

Plasmid

#104429

Purpose

super Ecliptic pHluorin in yeast expression vector

Depositor

Joel Kralj

Article

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

Super Ecliptic pHluorin

Use

Tags

Expression

Yeast

Mutation

Promoter

Tef1

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SAP130

Plasmid

#11241

Depositor

Robin Reed

Article

Das et al Mol Cell Biol. 1999 Oct . 19(10):6796-80

Insert

SAP130 (SF3B3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pWS3808

Plasmid

#185749

Purpose

Sub-array spacer 4/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 4/4

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EZ-L560

Plasmid

#173028

Purpose

Encodes OptoAMP2 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2_2

Plasmid

#118226

Purpose

Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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