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pBEL2003
Plasmid#139888PurposeExpression plasmid for LetAB with 5 ring (rings 12347) LetB (without TM region) mutantDepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(43-877; delta515-746)-6xHisPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2000
Plasmid#139885PurposeExpression plasmid for LetAB with 2 ring (rings17) LetB (without TM region) mutantDepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(43-877; delta161-747)-6xHisPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
U_(+36GFP)-Cre.ape
Plasmid#71749PurposeU+36GFP-Cre fusion expression in E. ColiDepositorInsertU_(pos36GFP)-Cre
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXC6 (human) HIS-tag pET
Plasmid#8565DepositorInsertHOXC6 (HOXC6 Human)
UseTagsHis and T7ExpressionBacterialMutationThis protein starts at an internal Met initially …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
EIF4A2
Plasmid#25259PurposeBacterial expression for structure determination; may not be full ORFDepositorInserteIF4A2:G22-E240 (EIF4A2 Human)
UseTagsHisExpressionBacterialMutationContains amino acids G22-E240PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
4IA9
Plasmid#51320PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5 (WDR5 Human)
UseTags6xHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
knob-s48i48
Plasmid#68391PurposeKnob protein fused to elastin-like polypeptide (S48I48)DepositorInsertKnob-S48I48
UseTagsKnobExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_H303A
Plasmid#67730PurposeExpresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged histidine 303 to alaninePromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_D230A
Plasmid#67725PurposeExpresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged aspartate 230 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N191A
Plasmid#67724PurposeExpresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged asparagine 191 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_K295A
Plasmid#67726PurposeExpresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged lysine 295 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 8, MG-26-21)
Plasmid#110745PurposeBacterial expression of human USP30 (construct 8)DepositorInsertUSP30 (USP30 Human)
UseTagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350D, I353E + insertion deletion: 64-357;…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
4PZI
Plasmid#61955PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMLL4:JMC054-D08:C226821 (KMT2B Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
HECW2
Plasmid#32875PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECW2 (HECW2 Human)
UseTagsHis tag (with TEV cleavage)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Aurein1.2_(+36GFP)-BirA
Plasmid#71752PurposeAurein1.2+36GFP-BirA fusion for E.Coli expressionDepositorInsertAurein1.2+36GFP-BirA
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
ITSN2 (3GF9)
Plasmid#25174PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITSN2:C1103-E1379 (ITSN2 Human)
UseTagsHisExpressionBacterialMutationContains amino acids C1103-E1379PromoterAvailable sinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
4QQG
Plasmid#61948PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP:JMC001-B06:C41570 (KAT5 Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
His10-PS-SNAPf-9E (XSB526)
Plasmid#78514PurposeConstruct for expressing His10 - SNAPf -9E in E. ColiDepositorInsertSNAPf
UseTagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutation9E added to the C-terminal of SNAPfPromoterAvailable sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPB41
Plasmid#32040PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEPB41 (EPB41 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationFERM domain only (amino acids 1-279)PromoterAvailable sinceJan. 13, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppj217(JBEI-13454)
Plasmid#89787Purposefor expression of N-8xHis-StrepII-MBP-3607DepositorInsert3607
UseTags8xHis-StrepII-MBPExpressionBacterialMutationPromoterAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
BRPF1 (3MO8)
Plasmid#28107PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRPF1:JMC014-E01:C44383 (BRPF1 Human)
UseTagsHis-tag with integrated TEV protease siteExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
T7-AglC-His6
Plasmid#89726PurposeExpresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.DepositorInsertAglC
UseTagsHis6 and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-AglK-His
Plasmid#89714PurposeExpresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.DepositorInsertAglK
UseTagsHis6 and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-U_(+36GFP)-Cre
Plasmid#71750PurposeHis-TEV-U+36GFP-Cre for E.Coli expressionDepositorInsertU_(pos36GFP)-Cre
UseTags6X His and TEV proteaseExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
PrbC10
Plasmid#24788DepositorInsertPrb
UseTagsHisExpressionBacterialMutationIle75Val, Asp83Asn, Leu122Pro, and Glu135LysPromoterAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
KIF1C-FHA
Plasmid#25549PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKIF1C-FHA (KIF1C Human)
UseTagsHisExpressionBacterialMutationFHA domain onlyPromoterAvailable sinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
CENTG3 (3IHW)
Plasmid#25159PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCENTG3:P126-K291 (AGAP3 Human)
UseTagsHisExpressionBacterialMutationContains P126-K291PromoterAvailable sinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
HOXA9 (murine) no-tags pET
Plasmid#8493DepositorInsertHoxA9 (Hoxa9 Mouse)
UseTagsExpressionBacterialMutationmakes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
SDCCAG10
Plasmid#25610PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSDCCAG10 (CWC27 Human)
UseTagsHisExpressionBacterialMutationCyclophilin_CeCYP16-Like Domain (residues 8-173)PromoterAvailable sinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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MALT1 (3K0W)
Plasmid#25306PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertmalt1.129.326 (MALT1 Human)
UseTagsHisExpressionBacterialMutationamino acid residues 128-326PromoterAvailable sinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
MALT1 (3BFO)
Plasmid#25216PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertmalt1.226.314 (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains amino acid residues 226-314 of Isoform 2PromoterAvailable sinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
MZB216
Plasmid#221766Purpose"Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorInsertrtxA
UseTagsExpressionMutationPromoterT7 PromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-MhCINS-6xHis
Plasmid#194304PurposeBacterial expression of MhCINS protein with a C terminal His-tag and transit peptide removed at N terminalDepositorInsertMhCINS
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseA-6xHis
Plasmid#214808PurposeExpression of 6xhis-tagged micrococcal nuclease A (MNaseA)DepositorInsertOmpA-Micrococcal nuclease A
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
R152C USP7 TRAF
Plasmid#227229PurposeExpresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationR152C; aa 62-205PromoterT7-promoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
G392A FL USP7
Plasmid#227217PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationG392APromoterT7-promoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
N418S FL USP7
Plasmid#227215PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationN418SPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
H111Q USP7 TRAF
Plasmid#227227PurposeExpresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationH111Q; aa 62-205PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
G392D FL USP7
Plasmid#227216PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationG392DPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
K420E FL USP7
Plasmid#227223PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationK420EPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L373F FL USP7
Plasmid#227220PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationL373FPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Y331H FL USP7
Plasmid#227218PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationY331HPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
P273H FL USP7
Plasmid#227221PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationP273HPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
M225I FL USP7
Plasmid#227219PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationM225IPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
F118L USP7 TRAF
Plasmid#227228PurposeExpresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationF118L; aa 62-205PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L469R FL USP7
Plasmid#227225PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationL469RPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
E345K FL USP7
Plasmid#227224PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationE345KPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only
