We narrowed to 15,323 results for: pET
-
Plasmid#191200PurposeFusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & Streptavidin fusion
Tags6xHis and Streptavidin core domain, amino acids 1…ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-MfnG-TEV-His
Plasmid#191114PurposeExpresses MfnG-TEV-His in E. coliDepositorInsertMfnG
TagsHis tagExpressionBacterialAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28.His.3C.86b.8D2Q.Tau2N4R
Plasmid#226400PurposeExpresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 8D2Q mutant 2N4R tau for recombinant protein production in bacteriaDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28.His.3C.86b.20D3Q.Tau2N4R
Plasmid#226401PurposeExpresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteriaDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-PKP3(305-797)
Plasmid#202599PurposeExpresses ARM domain of plakophilin3DepositorInsertplakophilin3 (PKP3 Human)
TagsHis8 tag with precision protease cleavage siteExpressionBacterialMutationDeleted amino acids 1 to 304Available SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)
Plasmid#187708PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-380Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT (492)
Plasmid#187709PurposeExpress his-tagged NEDD4L W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-miRFP(-5)-HO1
Plasmid#219424PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertsmiRFP682
HO1
TagsMGHHHHHGGExpressionBacterialMutationContains Ribosomal Binding Site for expression of…PromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-NPR4 (SA binding domain)
Plasmid#226885PurposeExpresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble formDepositorInsertThe Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
TagsHis tagExpressionBacterialMutationNPR4 SA-binding core (residues 373-516), with a s…PromoterT7Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a HsDHFR-bio-10His
Plasmid#193883PurposeExpresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequenceDepositorInserthuman dihydrofolate reductase (DHFR) (DHFR Human)
TagsHis tag and biotinylation sequenceExpressionBacterialMutationCodon optimizedPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-UBE3A iso 2 N terminus
Plasmid#185643Purposeexpression of His-tagged UBE3A isoform 2 1-87 in bacterial cellsDepositorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28b C7H1G6_6H N-terminal
Plasmid#223434PurposeC7H1G6 GNAT expression vector with N-terminal His6 tagDepositorInsertC7H1G6 GNAT
TagsHis6Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28b R6CZ24_6H C-terminal
Plasmid#223435PurposeR6CZ24 Thiolase expression vector with C-terminal His6 tagDepositorInsertR6CZ24 Thiolase
TagsHis6Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-Gal4-p53
Plasmid#171076PurposeExpresses Gal4-p53 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
TagsHis and p53(1-85)ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-IRSp53(pLxIS)
Plasmid#221336PurposeOverexpression of IRSp53 pLxIS domain in bacteriaDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-MAVS(pLxIS)
Plasmid#221335PurposeOverexpression of MAVS pLxIS domain in bacteriaDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-TRIF(Nterm)
Plasmid#221334PurposeOverexpression of TRIF Nterm domain in bacteriaDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-GMIP(pLxIS)
Plasmid#221337PurposeOverexpression of GMIP pLxIS domain in bacteriaDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-SpyTag-(AffiHER2)x3
Plasmid#216281PurposeThree affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate CatcherDepositorInsertSnoopTag-SpyTag-(AffiHER2)x3
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, and SpyTagExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL2/4sub
Plasmid#216746PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL3/4sub
Plasmid#216747PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL3 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5/4sub
Plasmid#216748PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet_OPTN-thrombin-GST
Plasmid#216843PurposeFor expression in E. coli of OPTN (Internal ref. SMC1789)DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-mNeonGreen-HMG-15b
Plasmid#206872PurposeExpress mNeonGreen-HMGDepositorInsertmNeonGreen-HMG
ExpressionBacterialPromoterT7 promoterAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421)
Plasmid#213619PurposeBacterial expression of SUMO-tagged rat syt1 (96-421)DepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB - 4A
Plasmid#213625PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB - 4ADepositorInsertsyt7 C2AB - 4A (Syt7 Mouse)
TagsSUMOExpressionBacterialMutationF167A, F229A, I298A, L361AAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2A-2A 2B
Plasmid#213626PurposeBacterial expression of SUMO-tagged mouse syt7 C2A-2A 2BDepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB-2A
Plasmid#213627PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB-2ADepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM43 p62 dUBA (1-389)
Plasmid#210426PurposeBacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage siteDepositorAvailable SinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)
Plasmid#205049PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)
TagsMGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1
Plasmid#205050PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L1
TagsGRRGKKSRK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2
Plasmid#205051PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L2
TagsGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3
Plasmid#205052PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L3
TagsGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-mTurquoise2-15b
Plasmid#206871PurposeExpress tTALE-mTurquoise2DepositorInsert7-mer tTALE-mTurquoise2
ExpressionBacterialPromoterT7 promoterAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-mNeonGreen-15b
Plasmid#206870PurposeExpress tTALE-mNeonGreenDepositorInsert7-mer tTALE-mNeonGreen
ExpressionBacterialPromoterT7 promoterAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-mKO2-15b
Plasmid#206869PurposeExpress tTALE-mKO2DepositorInsert7-mer tTALE-mKO2
ExpressionBacterialPromoterT7 promoterAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo-MCS
Plasmid#212725PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-MCS
Plasmid#212724PurposeBacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-MCS-His6
Plasmid#212723PurposeBacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning siteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo-GFP
Plasmid#212720PurposeBacterial expression of His6-CUbo-GFPDepositorInsertGFP
Tags6xHis-CUboExpressionBacterialAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24-95~236
Plasmid#208634Purposefor E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236DepositorInserthuman RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
Tags6xHis and FlagExpressionBacterialMutationonly aa 95~236, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
Tags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-GFP
Plasmid#212719PurposeBacterial expression of His7-NUbo-GFPDepositorInsertGFP
Tags7xHis-NUboExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-GFP-His6
Plasmid#212718PurposeBacterial expression of NUbo-GFP-His6DepositorInsertGFP
Tags6xHis and NUboExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-His6-TEV-Mms2
Plasmid#212716PurposeBacterial expression of His6-Mms2, His6-tag cleavable with TEV-proteaseDepositorInsertMms2
Tags6xHis-TEVExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAAA
Plasmid#202587PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAEA
Plasmid#202588PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, changed O…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(143-421)msGFP
Plasmid#209010PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209011PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
Tags6xHis and msfGFPExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…Available SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-HypaSpCas9-NLS-6xHis
Plasmid#207384PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHypaSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN692A, M694A, Q695A, H698APromoterT7Available SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only