We narrowed to 4,386 results for: ARA-2

1. pT7-AsCas12a_crRNA-site3 (RTW549)

   Plasmid

   #160138

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-EGFP-DmHPat-del57-499-dsRNAres_J

   Plasmid

   #146691

   Purpose

   Insect Expression of DmHPat-del57-499-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmHPat-del57-499-dsRNAres (Patr-1 Fly)
   

   Expression

   Insect

   Mutation

   one silent mutation A1758G compared to the sequen…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-DmHPat-dsRNAres_J

   Plasmid

   #146692

   Purpose

   Insect Expression of DmHPat-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmHPat-dsRNAres (Patr-1 Fly)
   

   Expression

   Insect

   Mutation

   two non silent, A479V, G568D and one silent mutat…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-HA-DmHPat-del500-684-dsRNAres_H

   Plasmid

   #146466

   Purpose

   Insect Expression of DmHPat-del500-684-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmHPat-del500-684-dsRNAres (Patr-1 Fly)
   

   Expression

   Insect

   Mutation

   two silent mutations compared to the sequence gi…

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH015

   Plasmid

   #80398

   Purpose

   Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

   Depositor

   Richard Murray

   Article

   Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

   Inserts

   Anti-scaffold-ffGFP-3xFLAG
   CusR (cusR )
   AraC (araC )
   

   Use

   Synthetic Biology

   Tags

   C-terminal 3xFLAG tag and C-terminal LZx domain

   Expression

   Bacterial

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB1alpha11_SulR

   Plasmid

   #186424

   Purpose

   transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Insert

   SulR
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB1alpha2_KanR_BastaR_Rb7_GFP

   Plasmid

   #186427

   Purpose

   optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Inserts

   KanR
   BastaR
   Rb7
   eGFP
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   CaMV 35S and Pnos

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBG-ttLbUV2

   Plasmid

   #231386

   Purpose

   LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

   Depositor

   Qi-Jun Chen

   Article

   Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

   Insert

   ttLbCas12a Ultra V2
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D156R and E795L

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-synP-FLEX-splitTVA-EGFP-B19G

   Plasmid

   #52473

   Purpose

   Expresses splitTVA-EGFP-B19G

   Depositor

   Ian Wickersham

   Article

   Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

   Has Service

   AAV1

   Insert

   TVA-P2A-EGFP-P2A-B19G
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   Synapsin-1

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-7aa-ActA-pEGFP-C1

    Plasmid

    #177406

    Purpose

    To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUBQ10:AtFLS2

    Plasmid

    #202186

    Purpose

    Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    Flagellin sensing 2 (FLS2 Mustard Weed)
    

    Tags

    HA

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    David Schaffer, John Flannery

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Mutation

    7mer insertion in the AAV2 cap

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 HRPT2 136X

    Plasmid

    #11049

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 136X (CDC73 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 HRPT2 227X

    Plasmid

    #11050

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 227X (CDC73 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pCAGGS-RaichuEV-Rac1(T17N)

    Plasmid

    #164902

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    T17N mutation in the Rac1 domain which make the s…

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-His8-aCat(660-906)

    Plasmid

    #203333

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-906 only

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-His8-aCat(660-865)

    Plasmid

    #203335

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-865 only

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-His8-aCat(660-885)

    Plasmid

    #203334

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-885 only

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pROS13

    Plasmid

    #107927

    Purpose

    kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-Puro-ARHGAP26

    Plasmid

    #69467

    Purpose

    Expresses ARHGAP26

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    ARHGAP26 (ARHGAP26 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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