Addgene

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pAc5.1A-Dm-miR-92a

Plasmid

#22767

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 92a (mir-92a Fly, primary transcript)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pMM114

Plasmid

#127211

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, IPT, sgRNA, Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-Ku70

Plasmid

#46957

Purpose

Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku70 (XRCC6 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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eGFP L202 Reporter

Plasmid

#119129

Purpose

Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

mCherry and eGFP

Use

Lentiviral

Tags

Expression

Mutation

eGFP L202S

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-NLuc_SV40:PuroR

Plasmid

#183045

Purpose

Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

NanoLuciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMM113

Plasmid

#127210

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, AtSTM, sgRNA, Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMM119

Plasmid

#127213

Purpose

BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

GFP, WUS2, sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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hRad51(K133R)

Plasmid

#125571

Purpose

Expresses the hRad51(K133R) protein in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

K133R

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HMGB2

Plasmid

#222532

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB2 (HMGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBABEpuro HA Brca1

Plasmid

#14999

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

Brca1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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Vimentin-pLJM5

Plasmid

#189868

Purpose

Expresses human Vimentin in mammalian cells

Depositor

Yoav Shaul

Article

Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

Insert

Vimentin

Use

Tags

m-Cherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

Plasmid

#211469

Purpose

Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ATAD3A (ATAD3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFG2

Plasmid

#87250

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Use

Tags

parS segments

Expression

Yeast

Mutation

Promoter

TEF

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pZZMP26

Plasmid

#231755

Purpose

Mammalian expression of TOP6BL

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

TOP6BL (Gm960 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-Myosin

Plasmid

#79995

Purpose

LC-Myosin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

LC-Myosin (Myl9 Mouse)

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TERF1

Plasmid

#222542

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of TERF1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

TERF1 (TERF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBabepuroBAF180

Plasmid

#41078

Depositor

Ramon Parsons

Article

Xia et al Cancer Res. 2008 Mar 15;68(6):1667-74.

Insert

BAF180 (PBRM1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

3 silent mutations: 3595 a to g; 4981 a to t; 587…

Promoter

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a SnoopTag-mEGFP-SpyTag

Plasmid

#72325

Purpose

Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SnoopTag-mEGFP-SpyTag

Use

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-KLF5

Plasmid

#40900

Depositor

Jin-Tang Dong

Article

Chen et al Oncogene. 2002 Sep 26;21(43):6567-72.

Insert

Kruppel-like factor 5 (KLF5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PCMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pJBJZ_003

Plasmid

#155358

Purpose

Rifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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