We narrowed to 2,847 results for: PHI-1

1. pT22Ubm4Eng2bKRPC(#493)

   Plasmid

   #184091

   Purpose

   cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

   Insert

   4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   4xMyc

   Expression

   Mutation

   ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWalium Htt19Q-mCherry

   Plasmid

   #201248

   Purpose

   allows the expression of the insert in Drosophila melanogaster

   Depositor

   Pedro Domingos

   Article

   Branco-Santos et al Hum Mol Genet. 2017 Oct 1;26(19):3763-37

   Insert

   Huntingtin (HTT Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSens-4OmeNB

   Plasmid

   #216231

   Purpose

   Expresses GFP in response to 4'-O-Methylnorbelladine

   Depositor

   Andrew Ellington, Simon d'Oelsnitz

   Article

   d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

   Inserts

   RamR-4NB2
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   K64T, L67M, C135D, S138G

   Promoter

   J23103 and Pramr

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMiR-HPV16-E7/E1-miR-375-m1-5

   Plasmid

   #53704

   Purpose

   Luciferase reporter assay for HPV16 E7/E1 with point mutation on miR-375 binding site

   Depositor

   Edward Chan

   Article

   Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

   Insert

   HPV16-E7/E1
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutation in miR-375 binding sites m1-5 (refer to …

   Promoter

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pT22U6CatalE4PCh(#767)

   Plasmid

   #184078

   Purpose

   cyclofen-inducible Catalase activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Insert

   CatalDeltaC-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes) + in vitro tra…

   Tags

   Expression

   Mutation

   CatalDeltaC: deleted 516-527; ERT2: G400V, M543A,…

   Promoter

   Zf-Ubi+SP6

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. C2 G10 png/GFP in casper4

   Plasmid

   #112970

   Purpose

   P element vector for transposon insertion of png-GFP into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Insert

   png-GFP (png Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-puro_G-CEPIA1er

   Plasmid

   #164590

   Purpose

   Stable integration and expression of G-CEPIA1er in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   G-CEPIA1er
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-IRES-Hygro_RGECO1.2

   Plasmid

   #164592

   Purpose

   Stable integration and expression of RGECO1.2 in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   R-GECO1.2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pECIA2

   Plasmid

   #47032

   Purpose

   This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

   Depositor

   Chris Garcia

   Article

   Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

   Type

   Empty backbone

   Use

   Secreted expression in drosophila culture

   Tags

   Fc(human IgG1), HRV 3C Protease Cleavage site, He…

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNA

    Plasmid

    #187063

    Purpose

    Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mhCMV

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRV DYRK2

    Plasmid

    #12568

    Depositor

    Anjana Rao

    Article

    Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

    Insert

    DYRK2 (DYRK2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

    Plasmid

    #146503

    Purpose

    Insect Expression of DmDCP1_327-372

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    DmDCP1_327-372 (Dcp1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA[DsxF].1026A

    Plasmid

    #112690

    Purpose

    express gRNA targeting DsxF under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[DsxF] (dsx Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-IRES-Hygro_GCaMP6s

    Plasmid

    #164591

    Purpose

    Stable integration and expression of GCaMP6s in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pECIA14

    Plasmid

    #47051

    Purpose

    This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

    Depositor

    Chris Garcia

    Article

    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

    Type

    Empty backbone

    Use

    Secreted expression in drosophila culture

    Tags

    Alkaline Phosphatase (human, placental), Flag, HR…

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST12.2 DYRK2 WT

    Plasmid

    #20005

    Depositor

    Anjana Rao

    Article

    Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

    Insert

    DYRK2 (DYRK2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hes5-Luc

    Plasmid

    #41724

    Depositor

    Raphael Kopan, Ryoichiro Kageyama

    Article

    Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

    Insert

    Hes5 Promoter (Hes5 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes5 promoter (-800 to +73)

    Promoter

    Hes5

    Available since

    Feb. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

19. Lck-mCherry-RGS

    Plasmid

    #112935

    Purpose

    Plasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)

    Depositor

    Dorus Gadella

    Article

    Reinhard et al Mol Biol Cell. 2017 Nov 7;28(23):3371-33

    Insert

    p115RhoGEF-RGS domain
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS mEya1 (CT#671)

    Plasmid

    #13971

    Depositor

    Cliff Tabin

    Article

    Heanue et al Mech Dev. 2002 Feb . 111(1-2):75-87.

    Insert

    Eya1 (Eya1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only