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Plasmid#41600DepositorInsertHis3
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-13Myc-His3MX6
Plasmid#41602DepositorInsertHis3
UseYeast genomic targetingTags13Myc, A. gossypii translation elongation factor …ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-Pa1
Plasmid#138475PurposeEnables high-efficiency recombineering in P. aeruginosaDepositorInsertsPapRecT
mutL E36K
UseTagsExpressionBacterialMutationE36KPromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term StrepII eGFP 3C
Plasmid#160683PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsStrepII eGFP 3CExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term GFP His8
Plasmid#160681PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsHis8 GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM3
Plasmid#154168PurposeEmpty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
hCas9_D10A
Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorInsertCas9_D10A
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Integrin alpha5-AHCys
Plasmid#27313DepositorInsertintegrin alpha 5 (ITGA5 Human)
UseTagsExpressionMammalianMutationintegrin alpha5 (1-954) + AHCysPromoterAvailable sinceMarch 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
Human LFA-1 alpha
Plasmid#8630DepositorInsertLFA1 alpha (ITGAL Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term His8 eGFP 3C
Plasmid#160684PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsHis8 eGFP 3CExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human ICAM-1
Plasmid#8632DepositorInsertICAM-1 (ICAM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA14 (MSW#503)
Plasmid#208069PurposeExpression of GFP-tagged hsRPA3 in human cellsDepositorInsertRPA3 (RPA3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-13Myc-kanMX6
Plasmid#39294DepositorInsertKanR
UseYeast genomic targetingTags13Myc, A. gossypii translation elongation factor …ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF1-alpha-V
Plasmid#27290DepositorInsertintegrin alpha V (ITGAV Human)
UseTagsExpressionMammalianMutationIsoform 2PromoterAvailable sinceMarch 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1328 LV EF1a-CD20 IRES-EGFP
Plasmid#201918Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD20DepositorInsertCD20
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3HA-kanMX6
Plasmid#39295DepositorInsertKanR
UseYeast genomic targetingTags3HA, A. gossypii translation elongation factor 1a…ExpressionMutationPromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-NLS-copGFP-EF1-BlastiS
Plasmid#132772PurposeSpills into the cytoplasm during nuclear envelope rupture.DepositorInsertNLS-copGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP
Plasmid#63215PurposeExpression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationPromoterCMV-EF1α hybrid (CEF)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GST-kanMX6
Plasmid#39293DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only