We narrowed to 14,168 results for: eng
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Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pPPC001
Plasmid#171138PurposeFor integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC011
Plasmid#171143PurposeExpression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmidDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC017.109
Plasmid#171145PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GYB
Plasmid#160927PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)DepositorInsertsBFP
sfGFP
ExpressionYeastPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1585588
Plasmid#62620PurposeePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
dihydroflavonol 4-reductase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
WT1 KI gRNA1
Plasmid#92312PurposeCRISPR-GFP-gRNA for cutting WT1DepositorAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)
Plasmid#197508PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…PromoterCMV and T7Available sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)
Plasmid#185916PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax C-terminal
Plasmid#137174PurposeExpresses the C-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_C-terminal
ExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax N-terminal
Plasmid#137173PurposeExpresses the N-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_N-terminal
ExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSXL101
Plasmid#247078PurposepZE12-luc containing MNX1 from Candida parapsilosis CBS604 and ubiC from E. coliDepositorInsertsMNX1
ubiC
AS
ExpressionBacterialAvailable sinceDec. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH72
Plasmid#202487PurposeExpresses the nanobody VHH72 with T7 inductionDepositorInsertnanobody VHH72
Tags6XHisExpressionBacterialPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH-GFPminimizer
Plasmid#202488PurposeExpresses the nanobody GFP minimizer with T7 inductionDepositorInsertnanobody GFP minimizer
Tags6XHisExpressionBacterialPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-VCAM1
Plasmid#202471PurposeExpresses the nanobody VCAM1 after phosphate depletionDepositorInsertnanobody VCAM1
Tags6X-HisExpressionBacterialPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-yibDp-Erv1p
Plasmid#202483PurposeExpresses sulfydryl oxidase (Erv1p) after phosphate depletionDepositorAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Spec-yibDp-Erv1p
Plasmid#212094PurposeExpresses sulfydryl oxidase (Erv1p) after phosphate depletionDepositorAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Spec-EV
Plasmid#212095Purpose(Empty Backbone) pCOLA-Spec Empty VectorDepositorTypeEmpty backboneExpressionBacterialAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVH615
Plasmid#169604PurposeTier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4 / PPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH617
Plasmid#169606PurposeTier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4 / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only