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Plasmid#136975PurposeGoldengate/Greengate module for genome editing, using the Sa guideRNA.DepositorInsertSa guide RNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_li_Tq2CFP_2NLS
Plasmid#136972PurposeGoldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linkerDepositorInsertTq2CFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGE_RT_Sa_sgRNA
Plasmid#136978PurposeGoldengate/Greengate module for genome editing, using the Sa guideRNA.DepositorInsertSa guide RNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_XVE
Plasmid#136970PurposeGoldengate/Greengate module for generation of beta-estradiole inducible genes.DepositorInsertXVE
UseTagsExpressionBacterialMutationBsaI site domestication 834 T > A,PromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGA_RT_Sa_Cas9
Plasmid#136962PurposeGoldengate/Greengate module for genome editing, using the SaCAS9.DepositorInsertSaCAS9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_biChromium
Plasmid#136974PurposeGoldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptideDepositorInsertP2A sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-METTL14-WT
Plasmid#141112PurposeOverexpression of WT METTL14 in mammalian cells.DepositorInsertHMETTL14 (METTL14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
Plasmid#51509PurposeCan be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoterDepositorHas ServiceAAV1InserttdTomato-T2A-SypEGFP
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCDH-METTL14-R298P
Plasmid#141113PurposeOverexpression of METTL14 mutant in mammalian cells.DepositorInsertHMETTL14 (METTL14 Human)
UseTagsExpressionMammalianMutationSubstitution - Missense, position 298, R➞PPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmiRA1-MYC
Plasmid#141121PurposeLentiviral overexpression of MYC in mammalian cells.DepositorInsertMYC (MYC Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-METTL14-WT
Plasmid#141118PurposeRetroviral overexpression of WT METTL14 in mammalian cells.DepositorInsertMETTL14 (METTL14 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-METTL14-R298P
Plasmid#141119PurposeRetroviral overexpression of mutant METTL14 in mammalian cells.DepositorInsertMETTL14 (METTL14 Human)
UseRetroviralTagsExpressionMammalianMutationSubstitution - Missense, position 298, R➞PPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Venus
Plasmid#136980PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Tq2CFP
Plasmid#136981PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry2_TagBFP
Plasmid#83031PurposeExpresses full length sfCherry2 and TagBFP fusion in mammalian cellsDepositorInsertsfCherry+full length_TagBFP
UseLentiviralTagsExpressionMammalianMutation3 mutations in sfCherry full length: E118Q, T128I…PromoterSFFVAvailable sinceJan. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXTm-PH domain
Plasmid#210326PurposeExpresses EGFP with PH domainDepositorInsertPH domain
UseFish expressionTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-SisTnpB1–ωRNA
Plasmid#228365PurposeExpresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cellsDepositorInsertSisTnpB1
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
PC1-29-9-2_GAL4-VHL
Plasmid#200434PurposeExpresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin LigaseDepositorInsertGAL4-VHL
UseTagsGAL4, VHLExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PC17-605-9-1_BRD4-BD2-VPR
Plasmid#200436PurposeExpresses the VPR transactivation domain linked to the BRD4(BD2) target proteinDepositorInsertBRD4(BD2)-VPR
UseTagsBRD4(BD2), VPRExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PC15-421-14-1_TRIM24-BD-VPR
Plasmid#200435PurposeExpresses the VPR transactivation domain linked to the TRIM24(BD) target proteinDepositorInsertTRIM24(BD)-VPR
UseTagsTRIM24(BD), VPRExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only