We narrowed to 10,315 results for: plasmids 101
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Plasmid#185918PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)
Plasmid#185802Purposeone plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2DepositorInsertcmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)
Plasmid#185800Purposeone plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1DepositorInsertcmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAMTA2-donor
Plasmid#132501PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorAvailable SinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAMTA2.1.0-gDNA
Plasmid#132456PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_Puro_ccdb_Ubc_rtTA
Plasmid#172130PurposeDestination vector for AAVS1 targetingDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterPGK1Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtnluc
Plasmid#184339PurposeTransformation of mitochondrial DNA in yeastDepositorInsertnanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
UseTagsExpressionYeastMutationPromoterAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HGS
Plasmid#39101PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mRuby3
Plasmid#244101PurposePlasma membrane targeted expression of green glucose sensor with non-responsive mRuby3DepositorInsertiGlucoSnFR2.mRuby3
UseAAVTagsIgG kappa leader and mRuby3ExpressionMutationPromoterAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR
Plasmid#113348PurposeA sgRNA expression plasmid for genome editing in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330-mcherry
Plasmid#98750PurposeCas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-(HA)hM4Di-W3SL
Plasmid#218759PurposeProvides AND intersectional (Cre+Flp) expression of HA-hM4DiDepositorInserthM4Di
UseAAVTagsHAExpressionMutationPromoterhSynAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-(HA)hM3Dq-W3SL
Plasmid#218758PurposeProvides AND intersectional (Cre+Flp) expression of HA-hM3DqDepositorInserthM3Dq
UseAAVTagsHAExpressionMutationPromoterhSynAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)
Plasmid#202553PurposeNontargeting control vector with TagBFP2DepositorInsertHumanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPuromycin-resistance gene in the pLV hU6-sgRNA hU…PromoterAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-dSa-VP64-p65(100-261)-RTA(125-190)
Plasmid#99679PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domainsDepositorArticleInsertdCas9
UseAAVTagsVP64-p65(101-261)-RTA(125-190)ExpressionMutationdead Cas9PromoterAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-luciferase-P2A-Neo
Plasmid#105621Purpose3rd generation lentiviral plasmid for expression of luciferase with Neo resistanceDepositorInsertLuciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEFSAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minCMV
Plasmid#211518PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minPro
Plasmid#211517PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minProAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minTK
Plasmid#211516PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minTKAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-3xFLAG-WPRE
Plasmid#127862PurposepAAV plasmid expressing 3xFLAG under the hSyn promoterDepositorInsert3xFLAG
UseAAVTagsExpressionMutationPromoterhSynAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only