We narrowed to 8,596 results for: Set

1. pAGK61

   Plasmid

   #228121

   Purpose

   Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT9679-sgRNA-BFP

   Plasmid

   #162988

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_SynP_DIO_tAKARα- T391A_WPRE

   Plasmid

   #196676

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   tAKARα-T391A
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWPI/hPLK2WT/Neo

   Plasmid

   #35385

   Depositor

   Anurag Tandon

   Article

   Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

   Insert

   hPLK2 (PLK2 Human)
   

   Use

   Lentiviral

   Tags

   EMCV IRES-Neomycin cassette and V5 epitope and Po…

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   April 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQmod2C-GG

   Plasmid

   #191347

   Purpose

   Clostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Plac-RFP (IPTG-inducible red fluorescent protein)
   

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLS.405

   Plasmid

   #187203

   Purpose

   Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA (wildtype)
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7/Lac

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL0341 (pTarget)

   Plasmid

   #200906

   Purpose

   Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   pTarget
   

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC2_4_dCas9_VPR_sgRNA

   Plasmid

   #163709

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-MBII85-5'SS mut

   Plasmid

   #67631

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Expression

   Mammalian

   Mutation

   mutation in 5'ss

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold

    Plasmid

    #226921

    Purpose

    Encoding SauriABE8e and sgRNA cassette on a single AAV

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    TadA8e, nSauriCas9
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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