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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 6971 - 6980 of 176427 results

  3. Gs(short)-CASE

    Plasmid
    #168124
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG1
    Expression
    Mammalian
    Mutation
    NLuc is inserted at N136/V137 within GNAS
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    168124-plasmid-map-sequence-id-332868

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  4. Gq-CASE

    Plasmid
    #168125
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F124/E125 within GNAQ
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    168125-plasmid-map-sequence-id-332869

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  8. pMH-Halo tag

    Plasmid
    #154144
    Purpose
    Gateway compatible mammalian expression plasmid for halo tag fusion proteins
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    Halo tag
    Expression
    Mammalian
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    154144-plasmid-map-sequence-id-309445

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  10. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Igor Ulitsky
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    141104-plasmid-map-sequence-id-298612

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Showing: 6971 - 6980 of 176427 results