Addgene

Showing: 61 - 70 of 11095 results

Results per page:

Sort results by:

pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

Plasmid

#175547

Purpose

Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

Plasmid

#175525

Purpose

Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag10 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

Plasmid

#169338

Purpose

Expression of the LT-Fucci(CA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(CA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

Plasmid

#225064

Purpose

Expression of HalM-modified HalA2 in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

HalA2

Use

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET12a-HAL

Plasmid

#47097

Depositor

Marina Ramirez-Alvarado

Insert

HAL

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

Plasmid

#169337

Purpose

Expression of the LT-Fucci(SCA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SCA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TREM2_Halo_N_allele

Plasmid

#178122

Purpose

Donor vector for endogenous tagging of human TREM2 at the N-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (TREM2 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GBA_Halo_C_allele

Plasmid

#178127

Purpose

Donor vector for endogenous tagging of human GBA at the C-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (GBA1 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HaloTag-ER-3

Plasmid

#186960

Purpose

ER lumen localizaing HaloTag

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CALR (CALR Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge