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Plasmid#48365Purpose3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsert3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDS68
Plasmid#48366PurposeGFP-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsertGFP-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHJ32
Plasmid#48361PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-mCh-bla
Plasmid#210260PurposeIntegrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
mCherry
UseTagsExpressionMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHJ33
Plasmid#48362PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDS69
Plasmid#48367PurposeFLAG-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsertFLAG-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx120-20xMS2-Hyg
Plasmid#210220PurposeIntegrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHJ17
Plasmid#48356PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHJ22
Plasmid#48358PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only