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Showing: 121 - 140 of 13545 results

  1. pPgiCas13b-gRNA-NT

    Plasmid
    #184835
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184835-plasmid-map-sequence-id-377028

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  2. pPlacPbuCas13b-gRNA-NT

    Plasmid
    #184838
    Purpose
    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184838-plasmid-map-sequence-id-373981

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  8. GFP-itis pBE-nt

    Plasmid
    #195343
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA
    Depositor
    Alexis Komor
    Article
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    Use
    CRISPR
    Tags
    Expression
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195343-plasmid-map-sequence-id-383225

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  9. pEXP5-NT/pT7 LasI

    Plasmid
    #193631
    Purpose
    Constitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LasI
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193631-plasmid-map-sequence-id-382852

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  10. pEXP5-NT/pT7 LuxI

    Plasmid
    #193626
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxI
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193626-plasmid-map-sequence-id-382174

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  11. pEXP5-NT/pLas eGFP

    Plasmid
    #193630
    Purpose
    Inducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    eGFP
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193630-plasmid-map-sequence-id-382855

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  12. pEXP5-NT/pLux eGFP

    Plasmid
    #193624
    Purpose
    Inducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    eGFP
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    pLux
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193624-plasmid-map-sequence-id-381997

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  13. pEXP5-NT/pT7 LuxR

    Plasmid
    #193625
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxR
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193625-plasmid-map-sequence-id-388599

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  14. pEXP5-NT/pT7 LasR

    Plasmid
    #193629
    Purpose
    Constitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LasR
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193629-plasmid-map-sequence-id-382851

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  17. pLV-NT-3-mCherry

    Plasmid
    #190201
    Purpose
    To express the mouse NT-3 tagged with the FP mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    NT-3
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190201-plasmid-map-sequence-id-374466

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  18. pCRT7 NT Topo arabinose

    Plasmid
    #73446
    Purpose
    Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides
    Depositor
    Jesse Rinehart
    Type
    Empty backbone
    Use
    Tags
    GST (N-terminal), 6xHis (C-terminal)
    Expression
    Bacterial
    Mutation
    Promoter
    pBad
    Available since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    73446-plasmid-map-sequence-id-264560

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Showing: 121 - 140 of 13545 results