Skip to main content
Addgene
Showing: 181 - 210 of 904 results

  1. pNSU299_SLA1

    Plasmid
    #166075
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SLA1 (SLA1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166075-plasmid-map-sequence-id-329130

    Enlarge

  2. pNSU299_PDR12

    Plasmid
    #166093
    Purpose
    Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    PDR12 (PDR12 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166093-plasmid-map-sequence-id-329121

    Enlarge

  3. pNSU299_TDH2

    Plasmid
    #166082
    Purpose
    Plasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    TDH2 (TDH2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166082-plasmid-map-sequence-id-329117

    Enlarge

  4. pNSU299_LYS9

    Plasmid
    #166085
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    LYS9 (LYS9 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166085-plasmid-map-sequence-id-329116

    Enlarge

  5. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

    Enlarge

  6. Peripherin/pAS2-1

    Plasmid
    #98135
    Purpose
    Peripherin (NM_006262) in DNA-binding domain (BD) vector, used for yeast-two hybrid
    Depositor
    Rita Shiang
    Insert
    Peripherin (PRPH Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98135-plasmid-map-sequence-id-191140

    Enlarge

  7. pTH341-SUP45(139-437)-GalBD

    Plasmid
    #29741
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 139-437 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 21, 2011
    Availability
    Academic Institutions and Nonprofits only
    29741-plasmid-map-sequence-id-13825

    Enlarge

  8. pTH339-SUP45(1-138)-GalBD

    Plasmid
    #29739
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 1-138 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    29739-plasmid-map-sequence-id-13823

    Enlarge

  9. pTH342-SUP45(276-437)-GalBD

    Plasmid
    #29742
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 276-437 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    29742-plasmid-map-sequence-id-13826

    Enlarge

  10. p404TEF1

    Plasmid
    #15972
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15972-plasmid-map-sequence-id-8457

    Enlarge

  11. p404ADH1

    Plasmid
    #15970
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15970-plasmid-map-sequence-id-8455

    Enlarge

  12. pGBK CUP1 Empty

    Plasmid
    #169710
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169710-plasmid-map-sequence-id-335105

    Enlarge

  13. p404GALS

    Plasmid
    #17435
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GALS promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17435-plasmid-map-sequence-id-8609

    Enlarge

  14. pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer

    Plasmid
    #58837
    Purpose
    Expresses GFP-Tagged Human PLC delta PH domain in yeast
    Depositor
    Tim Levine
    Insert
    2x Phospolipase C delta1 PH domain (Plcd1 Rat)
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    PHO5
    Available since
    Sept. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pNSU299_CUP1-1

    Plasmid
    #166086
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CUP1-1 (CUP1-1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166086-plasmid-map-sequence-id-330352

    Enlarge

  16. pNSU299_TDH3

    Plasmid
    #166084
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of TDH3 (TDH3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166084-plasmid-map-sequence-id-329123

    Enlarge

  23. p404CYC1

    Plasmid
    #15971
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    CYC1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15971-plasmid-map-sequence-id-8456

    Enlarge

  24. p404MET25

    Plasmid
    #17420
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    MET25 promoter is between SacI and XbaI restricti…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17420-plasmid-map-sequence-id-8595

    Enlarge

  25. p406MET25

    Plasmid
    #17421
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    MET25 promoter is between SacI and XbaI restricti…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17421-plasmid-map-sequence-id-8596

    Enlarge

  28. pNSU299_RPL39

    Plasmid
    #166078
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166078-plasmid-map-sequence-id-330349

    Enlarge

  29. pNSU299_YDR344C

    Plasmid
    #166072
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166072-plasmid-map-sequence-id-329603

    Enlarge

Showing: 181 - 210 of 904 results