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pYES2/NTA/Leu-LegA7ΔNAnk264

Plasmid

#216521

Purpose

Expression of LegA7 ΔNAnk264

Depositor

Ralph Isberg

Insert

LegA7 ΔNAnk264

Use

Tags

HIS-tag

Expression

Bacterial and Yeast

Mutation

LegA7 missing aa 264-361

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pYES2/NTA/Leu-LegA7ΔNAnk290

Plasmid

#216522

Purpose

Expression of LegA7 ΔNAnk290

Depositor

Ralph Isberg

Insert

LegA7 ΔNAnk290

Use

Tags

HIS-tag

Expression

Bacterial and Yeast

Mutation

LegA7 missing aa 290-361

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkG ZU5in-3xHA

Plasmid

#213730

Purpose

Mammalian cell expression vector for overexpression of AnkG ZU5in isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkG ZU5in (Ank3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkB ZU5ex-3xHA

Plasmid

#213732

Purpose

Mammalian cell expression vector for overexpression of AnkB ZU5ex isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkB ZU5ex (Ank2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkG ZU5ex-3xHA

Plasmid

#213731

Purpose

Mammalian cell expression vector for overexpression of AnkG ZU5ex isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkG ZU5ex (Ank3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Shank3 [N69/46R-2b]

Plasmid

#206675

Purpose

Mammalian Expression Plasmid of anti-Shank3 (Rat). Derived from hybridoma N69/46-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Shank3 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Shank3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_Halo-PanK

Plasmid

#197948

Purpose

Expression of FRET_control in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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Pan-Shank scFv [N23B/49]

Plasmid

#190542

Purpose

Mammalian Expression of Pan-Shank scFV. Derived from hybridoma N23B/49.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Shank (Rattus norvegicus) recombinant scFV (Shank2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-TalO8 (Blank-CoTrIP)

Plasmid

#178303

Purpose

Blank CoTrIP plasmid

Depositor

Brian Zid

Article

Chen et al Elife. 2022 Sep 15;11. pii: 76965. doi:

Type

Empty backbone

Use

Tags

NA

Expression

Yeast

Mutation

Promoter

NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR N52R (Shank3)

Plasmid

#175255

Purpose

SPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with N52R mutation from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-KANK3

Plasmid

#69238

Purpose

U6 driven SpCas9 sgRNA expression for KANK3 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

KANK3 sgRNA (KANK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Ankyrin-G (staining) [N106/43R]

Plasmid

#128635

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-G (staining) (Human). Derived from hybridoma N106/43.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Ankyrin-G (staining) (Human) recombinant mouse monoclonal antibody (ANK3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CRY2clust-mCherry-RANK-P2A-CIBNcaax

Plasmid

#208613

Purpose

Mammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)

Depositor

Takao Nakata, Tomohiro Ishii

Article

Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

Insert

CRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mouse, Mustard Weed)

Use

Tags

CAAX and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Ankyrin-G (blotting) [N106/20R]

Plasmid

#177495

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-G (blotting) (Human). Derived from hybridoma N106/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Ankyrin-G (blotting) (Homo sapiens) recombinant mouse monoclonal antibody (ANK3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Shank [N23B/49R]

Plasmid

#128637

Purpose

Mammalian Expression Plasmid of anti-Pan-Shank. Derived from hybridoma N23B/49.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Shank Pan recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Ankyrin [N388A/60R]

Plasmid

#128640

Purpose

Mammalian Expression Plasmid of anti-Pan-Ankyrin. Derived from hybridoma N388A/60.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Ankyrin Pan recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_COX8-Halo-PanK

Plasmid

#197949

Purpose

Expression of FRET_control in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-ecPanK

Use

Tags

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

Plasmid

#213969

Purpose

AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

Plasmid

#61590

Purpose

Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

Depositor

David Komander

Article

Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

Insert

TRABID (ZRANB1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-244 and aa 698-708.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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