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Plasmid#157592PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertCHL1 (CHL1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CHL1-Fc(DAPA)-AviTag-6xHis
Plasmid#157028PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCHL1 (CHL1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)
Plasmid#163066PurposeEntry vector for: ClopHensorN chloride sensor (cytosolic)DepositorInsertClopHensorN
UseGateway entry vectorTagstdTomatoExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP
Plasmid#179840Purposedonor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagschloride-conducting ChR (ChloC) and eGFPExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKD3_mCherry
Plasmid#187385PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRTDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
UseTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_1_001_psbH_5'Hom_Cr
Plasmid#235784Purpose5' Homology psbH in the M1 position used for genome integration at the psbH locusDepositorInsert5' Homology region of the psbH locus of Chlamydomonas reinhardtii chloroplast CC-125
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_1_004_psbA_5'Hom_Cr
Plasmid#235785Purpose5' Homology psbA in the M1 position used for genome integration at the psbA locusDepositorInsert5' Homology region of the psbA locus of Chlamydomonas reinhardtii chloroplast CC-125
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_1_007_petB_5'Hom_cr
Plasmid#235787Purpose5' Homology petB in the M1 position used for genome integration at the petB locusDepositorInsert5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Human HMG-CoA lyase like 1 (HMGCLL1)
Plasmid#52434Purposemammalian expression of human HMG-CoA lyase like 1 (HMGCLL1)DepositorInsertHMGCLL1 (HMGCLL1 Human)
UseTagsHISExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-67Sma
Plasmid#49342PurposeChlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic regionDepositorInsertEcoRI 5.8 kB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_5b_006_ftsH_3'UTR_Cr
Plasmid#235938PurposeftsH 3'UTR Chlamydomonas reinhardtiiDepositorInsertftsH 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_5b_001_psbA_3'UTR_Cr
Plasmid#235934PurposepsbA 3'UTR Chlamydomonas reinhardtiiDepositorInsertpsbA 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_5b_002_atpB_3'UTR_Cr
Plasmid#235935PurposeatpB 3'UTR Chlamydomonas reinhardtiiDepositorInsertatpB 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_5b_003_petL_3'UTR_Cr
Plasmid#235936PurposepetL 3'UTR Chlamydomonas reinhardtiiDepositorInsertpetL 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_5_041_ycf12_3'UTR_Cr
Plasmid#235927Purposeycf12 3'UTR Chlamydomonas reinhardtiiDepositorInsertycf12 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_5_045_rpoA_3'UTR_Cr
Plasmid#235930PurposerpoA 3'UTR Chlamydomonas reinhardtiiDepositorInsertrpoA 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_5_035_rps8_3'UTR_Cr
Plasmid#235923Purposerps8 3'UTR Chlamydomonas reinhardtiiDepositorInsertrps8 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_5_036_rps11_3'UTR_Cr
Plasmid#235924Purposerps11 3'UTR Chlamydomonas reinhardtiiDepositorInsertrps11 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_5_038_rps19_3'UTR_Cr
Plasmid#235925Purposerps19 3'UTR Chlamydomonas reinhardtiiDepositorInsertrps19 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only