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Plasmid#230831PurposeAAV cloning vector for Flpo dependent expression under the GFAP promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only
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pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE
Plasmid#218873PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2(no bind)
UseAAVTagsExpressionMammalianMutationS99A F102G R168PPromoterGFAPAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hGFAP-ND1-K139D-T2A-mCherry
Plasmid#178587PurposeExpression of NEUROD1-K139D and mCherry under the human GFAP promoterDepositorInsertNEUROD1-K139D (NEUROD1 Human)
UseAAVTagsExpressionMammalianMutationNEUROD1-K139DPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hGFAP-ND1-R111L-T2A-mCherry
Plasmid#178586PurposeExpression of NEUROD1-R111L and mCherry under the human GFAP promoterDepositorInsertNEUROD1-R111L (NEUROD1 Human)
UseAAVTagsExpressionMammalianMutationNEUROD1-R111LPromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB6-Gfap-T2A-tdTomato-FNF-DTA
Plasmid#140271PurposeB6J Gfap-T2A-tdTomato allele targeting vector, low copy numberDepositorInsertGfap (Gfap Mouse)
UseMouse TargetingTagsC-terminal fusion of T2A-tdTomatoExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB6-Gfap-T2A-td-sfGFP-FNF-DTA
Plasmid#137044PurposeB6J Gfap-T2A-td-sfGFP allele targeting vector, low copy numberDepositorInsertGfap (Gfap Mouse)
UseMouse TargetingTagsT2A-td-sfGFPExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR
Plasmid#154001PurposeVector expressed CasRx and sgRNA_Ptbp1 for AAV packageDepositorInsertCasRx, Ptbp1 sgRNAs
UseCRISPR and Mouse TargetingTagsFLAGExpressionMutationPromoterGFAP, U6Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2
Plasmid#178575PurposeLentiviral vector for GFAP::Opto-vTrapDepositorInsertCRY2PHR-P2A-CIBN-mCherry-VAMP2
UseLentiviralTagsExpressionMutationPromoterGFAPAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
anti-GFAP R416WT scFv [N206B/9] with sortase tag
Plasmid#204420PurposeMammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.DepositorInsertRecombinant mouse scFv targeting GFAP R416WT (Homo sapiens)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSYC-204
Plasmid#178964PurposeHuman GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationR79CPromoterZebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-201
Plasmid#178963PurposeHuman GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationPromoterzebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGfa2-nLac
Plasmid#53126PurposeExpression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains -2163 to +47 (the human gfa2 segment), w…Promoterhuman GFAPAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSYC-176
Plasmid#178961PurposeExpresses human GFAP fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-190
Plasmid#178962PurposeExpresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationR79CPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGfaABC1D-nLac
Plasmid#53131Purposeexpression of lacZ driven by human GfaABC1D promoterDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains ABC1D regions of Gfa2 promoter, -1757 to…Promoterhuman Gfa2Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGfa28-nLac
Plasmid#53130PurposeExpression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains the A and B regions (bp -1757 to -1489) …Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGfa2-EGFP
Plasmid#53128PurposeExpression of EGFP in mouse astrocytesDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsEGFP and mP1ExpressionMammalianMutationcontains -2163 to +47 (the human gfa2 segment), w…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f-AAV
Plasmid#182501PurposeGFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotorDepositorInsertER localized P450
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C2-SNAP
Plasmid#208794PurposeFor AAV production to express C2-SNAP in astrocytesDepositorInsertC2-SNAP (Mfge8 Mouse)
UseAAVTagsSNAP-tagExpressionMammalianMutationPromoterGFAP promoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C2m2-mKate
Plasmid#208793PurposeFor AAV production to express C2m2-mKate in astrocytesDepositorInsertC2m2-mKate (Mfge8 Mouse)
UseAAVTagsmKateExpressionMammalianMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterGFAP promoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only