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Plasmid#76428Purpose3rd generation lentiviral gRNA plasmid targeting human PRPF4BDepositorInsertPRPF4B
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 S1584F
Plasmid#234122PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationS1584FPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 S1584Y
Plasmid#234121PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationS1584YPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 V1862L
Plasmid#234123PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationV1862LPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 G1868R
Plasmid#234124PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationG1868RPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 WT TRP1
Plasmid#234120PurposeThe N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORFDepositorInsertPrp8
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRP.ABCA4 c.5461-10T
Plasmid#226553PurposeMinigene encoding the ABCA4 c.5461-10T mutationDepositorInsertABCA4 (ABCA4 Human)
UseTagsExpressionMammalianMutationc.5461-10T intronic mutationPromoterCAGAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-PrpsM
Plasmid#225282PurposeMini-Tn7 pGP-Tn7-Cm vector with constitutive PrpsM promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterConstitutive PrpsM promoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-PRPF39(1of2)
Plasmid#166199PurposeGateway entry vector for N. furzeri PRPF39DepositorInsertPRPF39(1of2)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_PRPF4:1-522
Plasmid#211109PurposeMammalian expression of full-length human PRPF4. N-terminal HiBiT tag.DepositorInsertPRPF4:M1-E522 (PRPF4 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_PRPF19:1-504
Plasmid#211173PurposeMammalian expression of full-length human PRPF19. C-terminal HiBiT tag.DepositorInsertPRPF19:M1-L504 (PRPF19 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_PRPF4:1-522
Plasmid#211148PurposeMammalian expression of full-length human PRPF4. C-terminal HiBiT tag.DepositorInsertPRPF4:M1-E522 (PRPF4 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_PRPF4:1-522
Plasmid#210896PurposeInsect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertPRPF4:M1-E522 (PRPF4 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRPS5A-PTP-3Xflag
Plasmid#189640PurposesTALED for plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 CaURA3 (pNTI592)
Plasmid#115431PurposeURA3-marked RPS28DepositorInsertsUseTagsExpressionYeastMutationKpnI site in intronPromoterAshbya gossypii and endogenousAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 SpHIS5 (pNTI663)
Plasmid#115433PurposeExpresses wild-type RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationMfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJU225 Prp8-TAP
Plasmid#111305PurposePrp8-TAPDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 AAAA
Plasmid#87600PurposeEncodes Prp5 AAAA for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationD136A, P137A, L138A, D139APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 E235A
Plasmid#87601PurposeEncodes Prp5 E235A for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationE235APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 N399D
Plasmid#87602PurposeEncodes Prp5 N399D for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN399DPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only