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Plasmid#163725PurposeLactate-inducible expression of green fluorescent protein encoding one amber codonDepositorInsertlldr gfp.1TAG
UseTagsExpressionBacterialMutationgfp.N39TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH627_pJH_LldR_PlldR-sfGFP.2TAG
Plasmid#163726PurposeLactate-inducible expression of green fluorescent protein encoding two amber codonsDepositorInsertlldr gfp.2TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,Y135TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH628_pJH_LldR_PlldR-sfGFP.3TAG
Plasmid#163727PurposeLactate-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertlldr gfp.3TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH721_pJH_LldR_PlldR-sfGFP.20TAG
Plasmid#163729PurposeLactate-inducible expression of green fluorescent protein encoding twenty amber codonsDepositorInsertlldr gfp.20TAG
UseTagsExpressionBacterialMutationgfp with all 20 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH727_pJH_LldR_PlldR-sfGFP.10TAG
Plasmid#163731PurposeLactate-inducible expression of green fluorescent protein encoding ten amber codonsDepositorInsertlldr gfp.10TAG
UseTagsExpressionBacterialMutationgfp with first 10 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH774_pJH_LldR_PlldR-sfGFP.1opal
Plasmid#163733PurposeLactate-inducible expression of green fluorescent protein encoding one opal codonDepositorInsertlldr gfp.1TGA
UseTagsExpressionBacterialMutationgfp with first leucine codon mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH775_pJH_LldR_PlldR-sfGFP.2opal
Plasmid#163734PurposeLactate-inducible expression of green fluorescent protein encoding two opal codonsDepositorInsertlldr gfp.2TGA
UseTagsExpressionBacterialMutationgfp with first two leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_035
Plasmid#109152PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFPDepositorInsertsmRFP
EGFP
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS_1N_400
Plasmid#170476PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O1, O-, Lambda t1 in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)
Plasmid#137110PurposeiFLinkC Linker cloningDepositorInsertMBP-CS(TEV)-StrepTag-II-AI(TVMV)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-pT7-sfGFP_RED20
Plasmid#135173PurposeExpresses sfGFP recoded in the RED20 genetic code off the T7 promoterDepositorInsertsfGFP_RED20
UseSynthetic BiologyTagsExpressionMutationRecoded ORF of sfGFP to only use one codon per am…PromotertaatacgactcactataAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2AGeneSwitch
Plasmid#125216PurposeArtificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGeneSwitch
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(1)_T2AGeneSwitch
Plasmid#125215PurposeArtificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGeneSwitch
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 AAAA
Plasmid#87600PurposeEncodes Prp5 AAAA for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationD136A, P137A, L138A, D139APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 E235A
Plasmid#87601PurposeEncodes Prp5 E235A for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationE235APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 N399D
Plasmid#87602PurposeEncodes Prp5 N399D for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN399DPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 TAG
Plasmid#87603PurposeEncodes Prp5 SAT motif to TAG for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationS446T, T448GPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-PRP2
Plasmid#87591PurposeEncodes Y2H Gal4 DNA binding domain for Prp2DepositorInsertPRP2 (PRP2 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 K335R
Plasmid#87299PurposeEncodes Y2H Gal4 activation domain for Hsh155 K335RDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationK335RPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 P369E
Plasmid#87277PurposeEncodes Hsh155 P369E for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationP369EPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only