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Plasmid#211580PurposeCMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selectionDepositorInsertPARP1 (PARP1 Human)
UseTagseGFPExpressionMammalianMutationContains E988K mutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1-Apex2–eGFP
Plasmid#211555PurposeA piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagsApex2-eGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1–eGFP
Plasmid#211552PurposeA piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-MCS
Plasmid#52908PurposeAedes aegypti polyubiquitin promoter followed by MCSDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAe aegptyi polyubiquitinAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7pol
Plasmid#59926PurposeExpression vector for T7 RNA polymerase geneDepositorInsertT7 RNA polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOpen-T4gene43
Plasmid#165524PurposePolymerase that replicates viral genomic DNA.DepositorInsertT4 DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Pwopol
Plasmid#165562PurposeDNA polymerase; additionally exhibits 3' to 5' exonuclease activity.DepositorInsertPwo DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-ECOpolA
Plasmid#165532PurposeDNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.DepositorInsertE. coli DNA Polymerase I
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-KTHEAQpol
Plasmid#165542PurposeExonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.DepositorInsertKlenTaq1 polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pOpen-T7gene1
Plasmid#165505PurposeHighly processive DNA-dependent RNA polymerase derived from T7 bacteriophageDepositorInsertT7 RNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Tlipol (exo-)
Plasmid#165523PurposeDNA polymerase; lacks 3' to 5' exonuclease activity.DepositorInsertTli DNA polymerase (exo-)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-9N7polA
Plasmid#165500PurposeThermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activityDepositorInsert9 degrees N-7 DNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Bstpol
Plasmid#165543PurposeFull length DNA polymerase from Bacillus stearothermophilus.DepositorInsertBst DNA Polymerase, Full Length
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-TthpolA
Plasmid#165533PurposeDNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).DepositorInsertTth DNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-3xpA
Plasmid#176505Purpose3x SV40 polyA sequencesDepositorInsert3x SV40 polyA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-2xpA
Plasmid#176504Purpose2x SV40 polyA sequencesDepositorInsert2x SV40 polyA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-4xpA
Plasmid#176506Purpose4x SV40 polyA sequencesDepositorInsert4x SV40 polyA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVL
Plasmid#64085PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain polymerase geneDepositorInsertVSV polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOpen-SP6rpol
Plasmid#165516PurposeDNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.DepositorInsertSP6 RNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-9N7polA (CT)
Plasmid#165502Purpose9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substratesDepositorInsert9 degrees N-7 DNA Polymerase chain terminating
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJY4092
Plasmid#113334PurposeVector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoterDepositorInsertcodon-optimized Hermes transposase
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
C95m
Plasmid#121001PurposeMoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7 RNA polymerase (Andy please check!)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
371-A
Plasmid#97026PurposeyORFDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-GBDpol
Plasmid#165501PurposeRobust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.DepositorInsertPyrococcus Sp. Heat-Stable DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-T5gene122
Plasmid#165544PurposePossesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.DepositorInsertT5 DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-K12polLF
Plasmid#165511PurposeDNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.DepositorInsertDNA Polymerase I, Large (Klenow) Fragment
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
371-U
Plasmid#97036PurposeMYFQSNA-yORFDepositorTypeEmpty backboneUseTagsMYFQSNAExpressionBacterialMutationPromoterT7Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIU211
Plasmid#44036DepositorInsertAGA1
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOpen-THEAQpolA
Plasmid#165512PurposeDNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.DepositorInsertTaq DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits