We narrowed to 10,918 results for: NSI

1. AAVS1-Bxb1-LP-TC

   Plasmid

   #183754

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox257
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDrVSP-IRES2-EGFP

   Plasmid

   #80333

   Purpose

   Expresses Danio rerio VSP with separated EGFP in mammalian cell

   Depositor

   Yasushi Okamura

   Article

   Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

   Insert

   Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDrVSP(C302S)-IRES2-EGFP

   Plasmid

   #80331

   Purpose

   Expresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cell

   Depositor

   Yasushi Okamura

   Article

   Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

   Insert

   Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C302S

   Promoter

   CMV

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-KP1

   Plasmid

   #206504

   Purpose

   AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   KP1 capsid
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   p5

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.Syn.GCaMP6f.WPRE.SV40

   Plasmid

   #100837

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-DsRed2

   Plasmid

   #112219

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   DsRed2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   DsRed2 contains a silent nucleotide substitution …

   Promoter

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-phiC31-LP-TC

   Plasmid

   #183755

   Purpose

   AAVS1 targeting construct with the phiC31 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   FRT-mCherry-attP-BleoR-F3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.CAG.GCaMP6f.WPRE.SV40

   Plasmid

   #100836

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV2, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Vamp2-pHluorin

   Plasmid

   #190151

   Purpose

   Expresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosis

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV

   Tags

   superecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CLYBL-Bxb1-LP-v2-TC

    Plasmid

    #194327

    Purpose

    CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

    Insert

    loxP-EBFP2-attP-BleoR-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57-RFP-P2A-MCS (Neo)

    Plasmid

    #89182

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40

    Plasmid

    #100845

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

    Plasmid

    #100833

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXJ-His-CD276

    Plasmid

    #232662

    Purpose

    transient expresses CD276 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CD276 (CD276 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid

    #100844

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX317-VRK1

    Plasmid

    #199643

    Purpose

    Expresses VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

    Plasmid

    #100835

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem

    Plasmid

    #229794

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

    Plasmid

    #229787

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; ZF10-NS3-p65
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. HotPETase_pET21b

    Plasmid

    #215407

    Purpose

    Expression construct for HotPETase gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    HotPETase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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