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  2. pTJV1Sc3-rpoB

    Plasmid
    #166983
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    RecT (recT E. coli)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pVvanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166983-plasmid-map-sequence-id-331334

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  5. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

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  14. pZmUbi-5'UTR

    Plasmid
    #154057
    Purpose
    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    Promoter and 5' UTR Ubiquitin (Zea mays)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    Sept. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154057-plasmid-map-sequence-id-313121

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  16. pTJV1Sc2-tetA

    Plasmid
    #166982
    Purpose
    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    CspRecT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pVanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166982-plasmid-map-sequence-id-331335

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Showing: 1381 - 1400 of 13333 results