We narrowed to 3,574 results for: golden gate

1. L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219690

   Purpose

   Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

   Plasmid

   #219688

   Purpose

   Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2BP/p35S (BFP, mCherry, NeonGreen)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219686

   Purpose

   Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MUT_S1-H1

   Plasmid

   #218394

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-responsive promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MUT_S7-H1

   Plasmid

   #218396

   Purpose

   3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-responsive promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. IBMc233

   Plasmid

   #161939

   Purpose

   Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PPhlF

   Available since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. IBMc050

   Plasmid

   #161938

   Purpose

   Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plux2

   Available since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. IBMc318

   Plasmid

   #161941

   Purpose

   Carries Golden Gate substitution insert for ER-LBD domain-insertion mapping

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-G-ER-LBD-S-SapI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGImC_V5-CSNK1A1

   Plasmid

   #92014

   Purpose

   Expresses V5-CSNK1A1, includes IRES-mCherry

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

    Plasmid

    #228636

    Purpose

    Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus A71 3C protease isoform B
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002

    Plasmid

    #228639

    Purpose

    Bacterial expression of the Coxsackievirus 33 isoform B 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Coxsackievirus 33 isoform B 3C protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease

    Plasmid

    #228644

    Purpose

    Bacterial expression of the D68 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus D68 3C protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAGM4723:Ble:Cas9

    Plasmid

    #112208

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

    Depositor

    Thomas Mock

    Inserts

    Cas9
    SheBle
    

    Use

    CRISPR; Diatom, phaeodactylum

    Tags

    Phaeodactylum fcp promoter and terminator and YFP

    Expression

    Mutation

    Phaeodactylum Fcp promoter

    Promoter

    Phaeodactylum Fcp

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAIP4_3CX2

    Plasmid

    #234221

    Purpose

    Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3CX2 (MYBPC3 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAIP4_4ACJ

    Plasmid

    #234212

    Purpose

    Heterologous protein expression of Wufb25h12 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4ACJ (oxr1a Zebrafish)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAIP4_1EYH

    Plasmid

    #234159

    Purpose

    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1EYH (Epn1 Rat)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. MUT_S1-spacer-H1

    Plasmid

    #218399

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline-responsive promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HS_NAM-B1

    Plasmid

    #154064

    Purpose

    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Inserts

    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    

    Use

    Cre/Lox and Synthetic Biology; Golden gate

    Tags

    Expression

    Bacterial and Plant

    Mutation

    The TtNAM-B1 gene sequence was domesticated to re…

    Promoter

    HvHSP17, OsAct, and ZmUbi

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. IBMc460

    Plasmid

    #161940

    Purpose

    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. IBMc180

    Plasmid

    #161942

    Purpose

    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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