We narrowed to 3,800 results for: golden gate

1. L0-T6 Circuit 1 GG1-Bxb1P-bbs1-PhiC31P-GG2

   Plasmid

   #219691

   Purpose

   Level 0 to construct history-dependent target T2PB

   Depositor

   Jennifer Nemhauser

   Insert

   GG1-Bxb1P-bbs1-PhiC31P-GG2
   

   Use

   Synthetic Biology

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. WT_S7-H1

   Plasmid

   #218393

   Purpose

   3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Expression

   Mammalian

   Promoter

   tetracycline-responsive promoter

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. UC16m

   Plasmid

   #121038

   Purpose

   MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gQi Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. UC17m

   Plasmid

   #121039

   Purpose

   MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gV1 Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. UCT1m

   Plasmid

   #121041

   Purpose

   MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   UCT-part guide RNA (Stanley Qi sequence)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. C108m

   Plasmid

   #121010

   Purpose

   MoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

   Depositor

   Richard Murray

   Insert

   mScarlet-I codon optimized with pdt3 tag
   

   Use

   Synthetic Biology

   Tags

   pdt3 mfLon degradation tag

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. C114m

   Plasmid

   #121013

   Purpose

   MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   dCas9 with no bbsI or BsaI sites
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. C103m

   Plasmid

   #121007

   Purpose

   MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

   Depositor

   Richard Murray

   Insert

   mScarlet-I codon optimized with LAA tag
   

   Use

   Synthetic Biology

   Tags

   LAA degradation tag for ssrA

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGIG_V5-PPP6C

   Plasmid

   #92013

   Purpose

   Expresses V5-PPP6C, includes IRES-Emerald

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   PPP6C (PPP6C Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAttBFHyg2

    Plasmid

    #244927

    Purpose

    attB Hyg Resistant plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion vector

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAIP4_2ESK

    Plasmid

    #234232

    Purpose

    Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2ESK (UBE2D2 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid

    #226429

    Purpose

    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

    Depositor

    Alon Goren

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAIP4

    Plasmid

    #233686

    Purpose

    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

    Depositor

    Christopher Bahl

    Insert

    Smt3 (SMT3 )
    

    Tags

    HisTag

    Promoter

    T7

    Available Since

    April 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease

    Plasmid

    #228637

    Purpose

    Bacterial expression of the Coxsackievirus 16 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Coxsackievirus 16 3C protease
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. ZIKV isolate PRVABC59 NS3 helicase domain

    Plasmid

    #228664

    Purpose

    Bacterial expression of the Zika virus NS3 helicase

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Zika virus NS3 helicase
    

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAIP4_4WDC

    Plasmid

    #234153

    Purpose

    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4WDC
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pRDB_031

    Plasmid

    #245331

    Purpose

    Cas12a CRISPRko all-in-one positive control; targets CD47, CD63

    Depositor

    John Doench, David Root

    Insert

    Cas12a, sgRNA CD47, CD63 (CD47 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDB_245

    Plasmid

    #245332

    Purpose

    Cas12a CRISPRko positive control guide; targets CD46

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD46 (CD46 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_837

    Plasmid

    #245328

    Purpose

    Cas9 CRISPRko positive control guide; targets CD55

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD55 (CD55 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDB_247

    Plasmid

    #245334

    Purpose

    Cas12a CRISPRko positive control guide; targets CD55

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD55 (CD55 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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