Addgene

Showing: 1401 - 1420 of 38160 results

Results per page:

Sort results by:

pEGFP-N1-mSNX27-H112A RNAi-resistant

Plasmid

#163621

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

H112A and RNAi resistance

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SS3 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145821

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS3 (Dlgap2 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SC27 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145819

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 nanobody (Rat) recombinant llama nanobody SC27 (Dlgap2 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SS2 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145820

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS2 (Dlgap2 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SS32 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145822

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS32 (Dlgap2 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Kir2.2 K+ channel [N124B/38R]

Plasmid

#140074

Purpose

Mammalian Expression Plasmid of anti-Kir2.2 K+ channel (Rat). Derived from hybridoma N124B/38.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kir2.2 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnj12 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SS44 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145824

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS44 (Dlgap2 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SS88 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145825

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS88 (Dlgap2 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF11-SFPQ(214-598)_quadruple_mutant

Plasmid

#135441

Purpose

Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

L535A, L539A, L546A, M549A

Promoter

T7

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Ataxin-1, 11NQ (Mouse) [N76/8R]

Plasmid

#128641

Purpose

Mammalian Expression Plasmid of anti-Ataxin-1, 11NQ (Mouse). Derived from hybridoma N76/8.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Ataxin-1, 11NQ (Mouse) recombinant mouse monoclonal antibody (Atxn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Flag-RNF125 Ring Mutant

Plasmid

#122046

Purpose

express RNF125 RM

Depositor

Ze'ev Ronai

Insert

RNF125 RM (RNF125 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ring mutant C37A, C40A

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-PARIS/ZNF746 [N196/16R]

Plasmid

#114475

Purpose

Mammalian Expression Plasmid of anti-PARIS/ZNF746 (Human). Derived from hybridoma N196/16.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-PARIS/ZNF746 (Homo sapiens) recombinant mouse monoclonal antibody (ZNF746 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-TRIP8b (exon 1a/5) [N291C/22R]

Plasmid

#114563

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (exon 1a/5) (Mouse). Derived from hybridoma N291C/22.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (exon 1a/5) (Mus musculus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-WRAP53beta-siRNA-resistant

Plasmid

#64680

Purpose

expresses siRNA resistant WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant

Promoter

CMV

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pRSETC-PITPbeta (splice variant 1) K61A

Plasmid

#58271

Purpose

bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_63/pRSETB

Plasmid

#51980

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-4GS

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 4

Plasmid

#45387

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 four point mutation (SERPINE1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutated from agacaaggttgt to acactaggatga

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 3

Plasmid

#45386

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 mutated at -723/-721

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-723/-721 mutated from tgt to aca

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 2

Plasmid

#45385

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 mutated at -730/-728

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutated at -730/-728 from aca to tgt

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant1

Plasmid

#45384

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI1 promoter -732/-721 mutated at -732/-731

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mutated -732/-731 from ag to tc

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only