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Showing: 2101 - 2130 of 5454 results

  2. pKL003

    Plasmid
    #98919
    Purpose
    Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    PROPS
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_J23118 - Strong Constitutive
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    98919-plasmid-map-sequence-id-189871

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  3. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Peter Schultz
    Article
    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31190-plasmid-map-sequence-id-418765

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  4. pBDC

    Plasmid
    #135188
    Purpose
    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    CmR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    cat
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135188-plasmid-map-sequence-id-273773

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  6. pRF0G-IodoY

    Plasmid
    #113645
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    IodoYRS and tRNA gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    113645-plasmid-map-sequence-id-217127

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  10. G-CaMP3

    Plasmid
    #22692
    Purpose
    a single-wavelength GCaMP2-based genetically encoded calcium indicator
    Depositor
    Loren Looger
    Article
    Tian et al Nat Methods. 2009 Dec;6(12):875-81.
    Insert
    G-CaMP3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N363D mutation (increased fluorescence)
    Promoter
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    22692-plasmid-map-sequence-id-241043

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  15. pTH847-gst-optsc

    Plasmid
    #96908
    Purpose
    Plasmid for yeast expression of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon optimised glutathione-S transferase
    Use
    Tags
    8xhistidine
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96908-plasmid-map-sequence-id-206073

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  17. pET-Trx-mSA2

    Plasmid
    #52320
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Use
    Tags
    FLAG, His, S tag, TEV site, and Thioredoxin
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52320-plasmid-map-sequence-id-84861

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  26. pNAS1B

    Plasmid
    #61968
    Purpose
    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    mCherry
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Serine 65 to Glycine (GGT), Serine 72 to Alanine …
    Promoter
    PBAD and PLtetO
    Available since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    61968-plasmid-map-sequence-id-101267

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  27. pCKmatBC

    Plasmid
    #138587
    Purpose
    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10
    Insert
    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    matB contains native GUG start codon
    Promoter
    Plac promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138587-plasmid-map-sequence-id-274478

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Showing: 2101 - 2130 of 5454 results