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pICH41421

Plasmid

#50339

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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TR-GFP

Plasmid

#80591

Purpose

Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL30009

Plasmid

#50301

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal Myc tag (4x Myc)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-talin1 head

Plasmid

#32856

Depositor

Anna Huttenlocher

Article

Simonson et al J Immunol. 2006 Dec 1;177(11):7707-14.

Insert

Talin-1 Head aa 1-433 (Tln1 Mouse)

Tags

Enhanced GFP

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pICSL50010

Plasmid

#50310

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal Myc tag (4x Myc)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p3692 HPV-16 E1

Plasmid

#10859

Depositor

Peter Howley

Article

Sakai et al J Virol. 1996 Mar . 70(3):1602-11.

Insert

HPV-16 E1 (E1 Human papillomavirus)

Expression

Mammalian

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only

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D1433 his3::LYS2 Disruptor Converter

Plasmid

#51676

Purpose

Yeast marker swap plasmid for converting HIS3 to LYS2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LYS2 (LYS2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFN18A-HaloTag-SpyCatcher

Plasmid

#206041

Purpose

HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

Depositor

Sergi Garcia-Manyes

Article

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Type

Empty backbone

Tags

HaloTag and SpyCatcher-8His

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLTR-RVG

Plasmid

#17577

Depositor

Jakob Reiser

Article

Mochizuki et al J Virol. 1998 Nov . 72(11):8873-83.

Insert

rabies virus glycoprotein (SAD B19 strain)

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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M4754 KanMX::HIS3 Disruptor Converter

Plasmid

#51684

Purpose

Yeast marker swap plasmid for converting KanMX3 to HIS3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

HIS3 (HIS3 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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SUMO_TwinStrep_Cas7-11_crRNA

Plasmid

#197612

Purpose

For protein purification of Cas7-11

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Cas7-11 with crRNA

Expression

Bacterial

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLSG (stra8)

Plasmid

#21619

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Stra8 promoter (Stra8 Mouse)

Use

Lentiviral

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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cavin-2-mEGFP

Plasmid

#27710

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

cavin-2 (CAVIN2 Human)

Tags

monomeric EGFP

Expression

Mammalian

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only

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pTRE-GFP-NMHC II-B

Plasmid

#10845

Depositor

Robert Adelstein

Article

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-B (MYH10 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hNanogP8

Plasmid

#19776

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

NanogP8 (NANOGP8 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-2

Plasmid

#24416

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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LbCas12a-gRNA_BbsI_CAM

Plasmid

#183222

Purpose

LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBPhsFlp2::PEST

Plasmid

#63171

Purpose

Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

hsFlp2::PEST

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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p1990 SV40-LMP1

Plasmid

#26654

Depositor

Bill Sugden

Article

Kaykas et al EMBO J. 2001 Jun 1. 20(11):2641-54.

Insert

Latent Membrane Protein 1 (LMP-1 Epstein-Barr Virus)

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pGAL4-CREB

Plasmid

#15221

Depositor

Ugo Moens

Article

Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

Insert

CREB (Creb1 Rat)

Tags

Gal4

Expression

Mammalian

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only