We narrowed to 1,479 results for: pbluescript

1. pT2-7xTcf-NLS-CNL-CP

   Plasmid

   #65715

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3-HPRT1-S104R-PdTK-mCh

   Plasmid

   #107276

   Purpose

   HPRT1 c.312C>A Munich donor vector with unilateral microhomology

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Inserts

   HPRT genomic DNA (HPRT1 Human)
   puro-deltaTK; CAG::mCherry
   

   Use

   Tags

   Expression

   Mutation

   c.306G>T, c.312C>A

   Promoter

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HOXA10 (human) bluescript

   Plasmid

   #8617

   Depositor

   Corey Largman

   Article

   Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

   Insert

   HOXA10 (HOXA10 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   improved Ribosomal binding site (NcoI site) at AT…

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pBS-f1(-)-ACTB-iTagRFP-T

   Plasmid

   #115916

   Purpose

   donor to insert iTagRFP-T at the ACTB locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   ACTB-TagRFP-T
   

   Use

   Donor template

   Tags

   TagRFP-T

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-f1(-)-SEC61B-dTomato

   Plasmid

   #115920

   Purpose

   donor to insert dTomato at the SEC61B locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SEC61B-dTomato
   

   Use

   Donor template

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBlue-hFX-HA

   Plasmid

   #14987

   Depositor

   Michael Ristow

   Article

   Shoichet et al Hum Mol Genet. 2002 Apr 1. 11(7):815-21.

   Insert

   human frataxin cDNA (FXN Human)
   

   Use

   Tags

   AU1 and HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

7. Mc4r->M71-IRES-tauGFP ACNF TV

   Plasmid

   #105072

   Purpose

   Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-333 of Mc4r and an IRES-tauGFP followed by ACNF cassette

   Depositor

   Peter Mombaerts

   Article

   Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.

   Insert

   Mc4r->M71-IRES-tauGFP-ACNF (Mc4r Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3-HPRT1-S104Rf-PdTK-mCh

   Plasmid

   #107277

   Purpose

   HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Inserts

   HPRT genomic DNA (HPRT1 Human)
   puro-deltaTK; CAG::mCherry
   

   Use

   Tags

   Expression

   Mutation

   c.306G>T, c.312C>A

   Promoter

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-HYGRO-P2A-mAID-mCLOVER-LRR1

   Plasmid

   #221546

   Purpose

   DONOR homology arm-containing repair template used to insert Auxin-Inducible Degron tags, mClover, and Hygromycin resistance markers to the N-terminus of LRR1

   Depositor

   Aga Gambus

   Article

   Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

   Insert

   LRR1 (LRR1 Human)
   

   Use

   Unspecified

   Tags

   HYGRO-P2A-mAID-mCLOVER

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Aug. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Slc1a3-CreERT2 Targeting Vector

    Plasmid

    #129409

    Purpose

    Gene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 locus in place of a STOP codon

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

    Insert

    Slc1a3-2A-CreERT2 Targeting Vector (Slc1a3 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-f1(-)-TOMM20-dTomato

    Plasmid

    #115922

    Purpose

    donor to insert dTomato at the TOMM20 locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    TOMM20-dTomato
    

    Use

    Donor template

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-f1(+)-GAPDH-IRES-dTomato

    Plasmid

    #115912

    Purpose

    donor to insert IRES-dTomato at the GAPDH locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    IRES-dTomato
    

    Use

    Donor template

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHA853 - mec-4p::Fynempty

    Plasmid

    #139210

    Purpose

    Control vector for Fyn kinase expression in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    deletion and frame shift to prevent Fyn expression

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHA851 - osm-10p::Fynempty

    Plasmid

    #139208

    Purpose

    Control vector for Fyn kinase expression in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    deletion and frame shift to prevent Fyn expression

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRepair-mTq2-CTNNB1

    Plasmid

    #153433

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    mTurquoise2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-FnCas12a-TLR-MCV1-gRNA

    Plasmid

    #117412

    Purpose

    FnCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    FnCas12a gRNA targeting TLR 2.0
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 289aa ELL2

    Plasmid

    #127266

    Purpose

    For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Three Mets (133, 138, 186) to Ileu. Contains 289…

    Promoter

    T7

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA tagged Xba ELL2 Met133, 138, 186 to Ileu

    Plasmid

    #127269

    Purpose

    For in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 Ileu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 Met133, 138, 186 to Ileu (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met133, 138, 186 to Ileu HA tag after M186

    Promoter

    T7

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA tagged Sph ELL2 Met133, 138, 186 to Ileu

    Plasmid

    #127271

    Purpose

    For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 Met133, 138, 186 to Ileu (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met133, 138, 186 to Ileu with HA tag after 8th Met

    Promoter

    T7

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. frameshift ELL2

    Plasmid

    #127257

    Purpose

    For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    XhoI site between the 1st &2nd Met was filled…

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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