We narrowed to 1,601 results for: tef

1. pRPL28(Q38P) SpHIS5 (pNTI664)

   Plasmid

   #115434

   Purpose

   Expresses Q38P RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Expression

   Yeast

   Mutation

   Q38P; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPL28(H39L) SpHIS5 (pNTI665)

   Plasmid

   #115435

   Purpose

   Expresses H39L RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Expression

   Yeast

   Mutation

   H39L; MfeI site in intron

   Promoter

   ashbya gospii TEF1 and endogenous

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. TIAM2_PDZ_1

   Plasmid

   #103952

   Purpose

   Protein expression and purification of TIAM2 PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   TIAM2 (TIAM2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAFMW-AthHEN1-CM

   Plasmid

   #104981

   Purpose

   Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Tags

   FLAG Myc

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPEE47

   Plasmid

   #139426

   Purpose

   Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mNeonGreen
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPEE46

   Plasmid

   #139425

   Purpose

   Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mRuby3
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. WT USP7 catalytic

   Plasmid

   #227201

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Article

   Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   aa 207-560

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWKS11

   Plasmid

   #139418

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mNeonGreen
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWKS12

    Plasmid

    #139419

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Superfolder GFP
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWKS4

    Plasmid

    #139411

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mCherry marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mCherry
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWKS2

    Plasmid

    #139410

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    GFP S65T
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-FOXM1

    Plasmid

    #153136

    Purpose

    Expression of Flag tagged human FOXM1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXM1 (FOXM1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROPseq-Guide-BSD

    Plasmid

    #216123

    Purpose

    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPOP-V5 (BTB)

    Plasmid

    #169721

    Purpose

    Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L186D, L190D, L193D, I217K

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA4/TO-GFP-SPOP (WT)

    Plasmid

    #197008

    Purpose

    Mammalian inducible expression vector for GFP-tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    -

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV14_NOP58

    Plasmid

    #73063

    Purpose

    expression clone for human NOP58 with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    NOP58 ribonucleoprotein (NOP58 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGWKS9

    Plasmid

    #139416

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mTurquoise2
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 HisC EXO1b

    Plasmid

    #68268

    Purpose

    Mammalian expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    CMV

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only