165,652 results

1. dCas9-hHDAC3

   Plasmid

   #98591

   Purpose

   3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCAS9-HDAC3 (HDAC3 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AP1

   Plasmid

   #179678

   Purpose

   Accessory plasmids

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [SD8] gIII
   

   Expression

   Bacterial

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGFP Cortactin

   Plasmid

   #50728

   Purpose

   mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   Cortactin (CTTN Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-n1-APP

   Plasmid

   #69924

   Purpose

   mammalian expression of human APP fused to EGFP

   Depositor

   Thomas Wassmer, Zita Balklava

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Insert

   APP (APP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1 NFAT3

   Plasmid

   #10961

   Depositor

   Toren Finkel

   Article

   Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

   Insert

   NFAT3 (NFATC4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. KRAB-dCas9

   Plasmid

   #112195

   Purpose

   KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   KRAB (ZNF10 Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-KRAB-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_CD40_WT

   Plasmid

   #82198

   Purpose

   Gateway Donor vector containing CD40 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CD40 (CD40 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-puro U6 sgRNA BfuAI stuffer

   Plasmid

   #50920

   Purpose

   U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS SK mCherryROSAegfp

   Plasmid

   #54322

   Purpose

   Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   mCherry and EGFP
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-3'3xFlag

    Plasmid

    #208616

    Purpose

    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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