We narrowed to 7,358 results for: Ank

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pCAG-rox-inv[Talpha1-iCre-pA]-rox-mNeonGreen-hCentrin1

Plasmid

#196892

Purpose

Neuron-specific expression of human (h)Centrin fused to mNeonGreen. Used in combination with Talpha1-Dre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mNeonGreen-hCentrin1 (CETN1 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-hCentrin1

Plasmid

#196891

Purpose

Neuron-specific expression of human (h)Centrin fused to 2xHA-tags

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

2xHA-hCentrin1 (CETN1 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mOrange2-rCM1

Plasmid

#196861

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2-rCM1 (Nedd1 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Akna-mOrange2

Plasmid

#196865

Purpose

Expression of the centrosomal protein Akna fused to mOrange2

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akna-mOrange2 (Akna Mouse)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFRSF11A-COMP5AP-AviTag-9xHis

Plasmid

#157153

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF11A (TNFRSF11A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Neo

Plasmid

#186672

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Neomycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.25_S6

Plasmid

#173960

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 6 (S6) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.35_S1

Plasmid

#173954

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 1 (S1) from SOX9 enhancer cluster EC1.35

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.35_S1-2

Plasmid

#173953

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer regions 1 and 2 (S1 and S2) from SOX9 enhancer cluster EC1.35

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del12

Plasmid

#173984

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #12

Use

Luciferase

Mutation

Tiled deletion #12

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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