We narrowed to 896 results for: inha

1. pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

   Plasmid

   #35502

   Purpose

   AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

   Plasmid

   #35509

   Purpose

   AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV5 and AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8-PHF19

   Plasmid

   #68864

   Purpose

   GW entry vector for PHF19

   Depositor

   Adrian Bracken

   Article

   Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

   Insert

   PHF19 (PHF19 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pETM6-G6-vioABE

   Plasmid

   #73438

   Purpose

   Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWPXL-Klf4

   Plasmid

   #36981

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 6, 2012

   Availability

   Academic Institutions and Nonprofits only

6. hSyn-somAion-citrine

   Plasmid

   #192570

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Article

   Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

   Insert

   somAion
   

   Use

   AAV

   Tags

   Citrine - soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE4938

   Plasmid

   #107527

   Purpose

   Expresses Fn crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and human U6

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE4495

   Plasmid

   #80338

   Purpose

   mammalian expression MbCpf1 nuclease and Mb crRNA

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Mb crRNA
   MbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

   Plasmid

   #69833

   Purpose

   Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyTag-C1C2
   

   Use

   Lentiviral

   Tags

   Trafficking signal and mCherry

   Expression

   Mammalian

   Mutation

   Inserted SpyTag after the signal peptide of C1C2.

   Promoter

   CamKIIa

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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