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Plasmid#172095PurposecDNA of CER11/CPL2 cloned into pET28a for protein expressionDepositorInsertCPL2 (CPL2 Mustard Weed)
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28_6xHis-PARP1delZn1-L713F
Plasmid#173937PurposeBacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn1-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 1-96; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3-L713F
Plasmid#173934PurposeBacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn3-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 214-373; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G871L
Plasmid#174798PurposeBacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-G871L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationG871L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn2
Plasmid#174805PurposeBacterial expression of isolated PARP1 Zn2 domainDepositorInsertPARP1-Zn2 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn3
Plasmid#174806PurposeBacterial expression of isolated PARP1 Zn3 domainDepositorInsertPARP1-Zn3 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A870L
Plasmid#174797PurposeBacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-A870L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationA870L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-K893I
Plasmid#174799PurposeBacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)DepositorInsertPARP1-K893I (PARP1 Human)
UseTags6xHisExpressionBacterialMutationK893I, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET28_hTLN2-head-LBD
Plasmid#164837PurposeExpression of human talin2 head mutantDepositorInsertTLN2 (TLN2 Human)
UseTagsHis8ExpressionBacterialMutationLipid binding deficient mutationsPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only