We narrowed to 1,254 results for: sumo

1. pcDNA5-FRT-TO-mCherry-N

   Plasmid

   #210271

   Purpose

   N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3flag-RanGAPdeltaN

   Plasmid

   #25104

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   RanGAP1 deleted N-term domain (RANGAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-203.

   Promoter

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pET28a-HsSENP6

   Plasmid

   #71469

   Purpose

   Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 6 (SENP6 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pRpr150-LUC

   Plasmid

   #85700

   Purpose

   luciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)

   Depositor

   Giannino Del Sal, Licio Collavin

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   RpR 150 enhancer and hsp70 minimal promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   RpR 150 enhancer and hsp70 minimal promoter

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pAc-HT-Dmp53

   Plasmid

   #85698

   Purpose

   expression of Drosophila p53 in Drosophila cells

   Depositor

   Giannino Del Sal, Licio Collavin

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   Dmp53 (p53 Fly)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pAc-HT-Dmp53KRKR

   Plasmid

   #85699

   Purpose

   expression of Drosophila p53 KRKR mutant in Drosophila cells

   Depositor

   Giannino Del Sal, Licio Collavin

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   Dmp53 (p53 Fly)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   K26R K302R

   Promoter

   Ac5

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

7. MZB160

   Plasmid

   #217841

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   L8K, Q71L

   Promoter

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOP823

   Plasmid

   #235718

   Purpose

   Protein expression of His6-SUMO-HsRAB5A (Q79L C19S,C63S) 1-212 in bacteria for maleimide conjugation

   Depositor

   Roger Williams

   Article

   Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

   Insert

   RAB5A (RAB5A Human)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Q79L, C19S, C63S, aa 1-212

   Promoter

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. MZB224

   Plasmid

   #226998

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   T31N

   Promoter

   T7

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MZB222

    Plasmid

    #221772

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    ArfGEF1 (ARFGEF1 Human)
    

    Use

    Tags

    10xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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