We narrowed to 2,368 results for: pcas9

1. pBBR-Cas9ΔyqhD-HA500

   Plasmid

   #220369

   Purpose

   A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

   Depositor

   Yilan Liu

   Inserts

   SpCas9
   sgRNA for yqhD in P. putida KT2440
   

   Use

   CRISPR; Broad host range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-ABE-P48R-UGI

   Plasmid

   #161817

   Purpose

   Expresses ABE-P48R-UGI in mammalian cells

   Depositor

   Sangsu Bae

   Insert

   bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PX458-AAVS1

   Plasmid

   #113194

   Purpose

   Encodes gRNA for human AAVS1

   Depositor

   Adam Karpf

   Insert

   AAVS1 (PPP1R12C, AAVS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PP7 guide

   Plasmid

   #86168

   Purpose

   Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   PP7 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLdCN2

   Plasmid

   #125186

   Purpose

   Expresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al Methods Mol Biol. 2020;2116:199-224. doi

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pECO200

   Plasmid

   #169765

   Purpose

   Express SpCas9 and Basta resistance gene

   Depositor

   Sang-Gyu Kim

   Article

   Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

   Type

   Empty backbone

   Use

   Plant binary vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S, AtU6

   Available Since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pECO300

   Plasmid

   #169766

   Purpose

   Express SpCas9 and Kanamycin resistance gene

   Depositor

   Sang-Gyu Kim

   Article

   Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

   Type

   Empty backbone

   Use

   Plant binary vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S, AtU6

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBRC1

   Plasmid

   #183064

   Purpose

   Base editing plasmid for Pseudomonas chlororaphis

   Depositor

   Xuehong Zhang

   Article

   Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

   Insert

   eSpCas9ppD10A
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCP

   Plasmid

   #204743

   Purpose

   Cas9-2A-Puro expression vector

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGB

    Plasmid

    #204744

    Purpose

    gRNA-Bsd expression vector

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ABE-RA1.0

    Plasmid

    #208297

    Purpose

    Expresses ABE-RA1.0 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA1.0-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D108G

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ABE-RA1.1

    Plasmid

    #208298

    Purpose

    Expresses ABE-RA1.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA1.1-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D108G, K161N

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GluA2 (px330:2-guideCas9)

    Plasmid

    #169420

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GluA1 (px330:2-guideCas9)

    Plasmid

    #169417

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p426*-SpSgH

    Plasmid

    #136993

    Purpose

    Helper plasmid for SpCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ABE-RA2.1

    Plasmid

    #208300

    Purpose

    Expresses ABE-RA2.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA2.1-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P48A, R51H, D108G, K110R, H122R, M126I, N127K

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCM4.4

    Plasmid

    #217648

    Purpose

    an ermE*p-cas9-driven expression, as an alternative plasmid

    Depositor

    Huimin Zhao

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    Codon Optimized SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KO plasmid

    Plasmid

    #135970

    Purpose

    Expresses SpCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge